Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 215-231-4 | CAS number: 1314-35-8
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-07-01 to 2002-08-20
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Tungsten trioxide
- EC Number:
- 215-231-4
- EC Name:
- Tungsten trioxide
- Cas Number:
- 1314-35-8
- Molecular formula:
- O3W
- IUPAC Name:
- trioxotungsten
- Details on test material:
- - Name of test material (as cited in study report): Tungsten oxide (WO3)
- Physical state: Yellow to greenish powder
- Analytical purity: 99.88%
- Purity test date: 2002-05-28
- Stability under test conditions: 5 years
- Storage condition of test material: Ambient temperature, dry
- Melting point: Approx. 1473 degrees C
- pH: 6.1 (aqueous suspension, 100 g/L)
- Solubility in water: < 10 mg/L WO3
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Charles River Wiga
- Weight at study initiation: 2.1-2.2 kg
- Housing: Individual caging in metal wire cages (79 cm x 59 cm bottom area, 38 cm height, with a wooden rest shelf, 15 cm x 59 cm)
- Diet (eg ad libitum): Altromin 2023 diet for rabbits, ad libitum. Hay- briquettes were offered additionally as a dietary supplement.
- Water (e.g. ad libitum): Tap water from an automatic water system, ad libitum
- Acclimation period: 5 days (one animal), 12 days (two other animals)
ENVIRONMENTAL CONDITIONS
- Temperature (°C): average of 19.4 °C
- Humidity (%): average of 66.7%
- Air changes (per hr): 12/hr
- Photoperiod (hrs dark / hrs light): 12 dark/12 light (6 am to 6 pm)
IN-LIFE DATES: From: 2002-07-01 To: 2002-07-12
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: The left eyes remained untreated and served as a control
- Amount / concentration applied:
- The approximate equivalent of 0.1 mL (~100 mg) of the test substance was administered per animal into the conjunctival sacs of the right eyes by gently pulling the lower lids away from the eyeballs for instillation. The eyes were held closed for about one second to prevent a loss of test substance.
- Observation period (in vivo):
- 72 hours
- Number of animals or in vitro replicates:
- 3 females
- Details on study design:
- SCORING SYSTEM: Scoring scheme for ocular lesions- examined the whole eye, the corneae, irises, and conjunctivae
TOOL USED TO ASSESS SCORE: otoscope lamp
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 3
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- Both the untreated eyes and the eyes treated with the test substance, for all three animals, were normal at each observation point.
- Other effects:
- No general toxic effects of the test substance were observed.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The administration of 100 mg of the test substance to the eyes of female rabbits, resulted in no signs of eye irritation. According to the Directive 2001/59/EC, the test substance is not "irritating to eyes."
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
