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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vivo

Currently viewing:

Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Evaluation of a three-exposure mouse bone marrow micronucleus protocol: Results with 49 chemicals.
Author:
Shelby, M.D., Erexson, G.L., Hook, G.J. and Tice, R.R.
Year:
1993
Bibliographic source:
Environmental and molecular mutagenesis 21: 160-179

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
GLP compliance:
no
Type of assay:
micronucleus assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Bis(2-ethylhexyl) adipate
EC Number:
203-090-1
EC Name:
Bis(2-ethylhexyl) adipate
Cas Number:
103-23-1
Molecular formula:
C22H42O4
IUPAC Name:
bis(2-ethylhexyl) adipate
Details on test material:
Supplier: NTP chemical repository (Radian Corporation, Austin, TX).

Test animals

Species:
mouse
Strain:
B6C3F1
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
Corn oil.
Duration of treatment / exposure:
Three consecutive days.
Frequency of treatment:
Three daily treatments.
Post exposure period:
A single sample was taken 24 hours after the final treatment (injection).
Doses / concentrations
Remarks:
Doses / Concentrations:
375, 750, 1500, 2000 mg/kg
Basis:
analytical conc.
No. of animals per sex per dose:
5 male mice per dose.
Control animals:
yes, concurrent vehicle
Positive control(s):
7,12-dimethylbenzanthracene (DMBA).

Examinations

Tissues and cell types examined:
Tissue: bone marrow.
Cells: erythrocytes.

Results and discussion

Test results
Sex:
male
Genotoxicity:
negative
Toxicity:
no effects
Vehicle controls validity:
valid
Negative controls validity:
valid
Positive controls validity:
valid

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative