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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
02 - 16 Aug 1996
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
Version / remarks:
Cited as Directive 87/302/EEC, part C, p. 95 (Toxicity for earthworms: Artificial soil test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on preparation and application of test substrate:
- Method of mixing into soil (if used): The test substance was directly added to the soil. The test substance -soil mixture was vigorously shaken.
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
- Source: eisenia GmbH und Co., Wiesbaden, Germany
- Age at test initiation: >= 2 months
- Weight at test initiation: 301 - 511 mg
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d
Test temperature:
20 +/- 2°C
pH:
initial: 6.5-6.8
Moisture:
37 - 40%
Details on test conditions:
- Earthworms were exposed to DEHA amended quartz sand and soil
- No. of organisms per concentration: 40
- Light intensity: ca. 400 lux
Nominal and measured concentrations:
- Test concentrations nominal: 0, 102, 171, 301, 550, 1000 mg/kg d.w.
Reference substance (positive control):
yes
Remarks:
chloracetamid
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
865 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 550-1000 mg/kg
Duration:
7 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
550 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
LC100 (14d) > 1000 mg/kg
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Relevant effect levels: 14d-EC50 = 30 - 100 mg/L
Validity criteria fulfilled:
yes
Conclusions:
An acute toxicity test performed according to EU method C.8 using Eisenia foetida as test organism revealed toxic effects of DEHA to earthworms resulting in a 14d-LC50 of 865 mg/kg.
Executive summary:

The study was performed according to EU method C.8 using Eisenia foetida as test organism. Test substance was directly added to the artificial soil to prepare nominal test concentrations between 102 and 1000 mg/kg d.w. Mortality was recorded after 7 and 14 days exposure time. Whereas no earthworm died in the control and test concentrations up to 500 mg/kg, 17 animals (out of 40) died in the 1000 mg/kg treatment. Thus, the 14d-LC50 is determined to be 865 mg/kg d.w. based on nominal concentrations.

Description of key information

LC50(14d) = 865 mg/kg d.w. (nominal) for Eisenia foetida (EU method C.8)

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
865 mg/kg soil dw

Additional information

One study investigating the short-term toxicity of Bis(2-ethylhexyl) adipate (CAS 103-23-1) to soil macroorganisms is available. The study was performed according to EU method C.8 using Eisenia foetida as test organisms (Huels 1996). Different nominal concentrations between 100 and 1000 mg/kg d.w. were tested. Mortality was recorded after 7 and 14 days exposure time. Whereas no earthworm died in the control and test concentrations up to 500 mg/kg, 17 animals (out of 40) died in the 1000 mg/kg treatment. Thus, the 14d-LC50 is determined to be 865 mg/kg d.w. based on nominal concentrations.

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