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Toxicity to aquatic algae and cyanobacteria

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Description of key information

Toxicity to aquatic algae - OECD 201 – EC50: 2.2 mg/l, 72h - PARAD consortium, NOACK, 2010, D. Scheerbaum)

Key value for chemical safety assessment

EC50 for freshwater algae:
2.2 mg/L

Additional information

Toxicity to aquatic algae - OECD 201 – EC50: 2.2 mg/l, 72h - PARAD consortium, NOACK, 2010, D. Scheerbaum)

The toxicity of the test item(batch number: S700040031)to the unicellular freshwater green algaDesmodesmus subspicatuswas determined according to the principles of OECD 201 atDr.U.Noack-Laboratorienin 31157 Sarstedt, GermanyfromMay 24 to June 09, 2010 with the definitive exposure phase of the study fromMay 25 to 28, 2010. 

The study was conducted under static conditions with an initial cell density of approximately 2 – 5 x 103cells/mL.Based on a preliminary test 5concentration levels were tested in a geometrical series with a dilution factor of √10, nominal: 0.100 - 0.320 - 1.00 - 3.20 - 10.0 mg/Lcorresponding to geometric mean measured test item concentrations of 0.0718 - 0.289 - 0.917 - 2.87 - 9.52 mg/L.Three replicates were tested for each test item concentration and six replicates for the control.Environmental conditionswere determined to be within the acceptable limits.

The concentrations of the test itemwereanalysed at all concentration levels after 0 and 72 h via LC-MS/MS analysis. At test start the recoveries of the test item were in the range of 102 to 111 % of the nominal values and at test end in the range of 47 to 86 % of the nominal values. All effect values are given based on geometric mean measured concentrations of the test item.The ERC50 after 72 h was determined to be: ERC50: 2.2 mg/l (based on geometric mean measured test item concentrations [mg/L])

 

Evaluation: valid without restrictions, Klimisch-I , Key study

Assessment: The test item is toxic to algae.