Registration Dossier
Registration Dossier
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EC number: 284-515-8 | CAS number: 84929-31-7 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Citrus limonum, Rutaceae.
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Read across from orange oil 96h-EL50: 5.65 mg/l (loading rate, WAF)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 5.65 mg/L
Additional information
The acute toxicity of lemon oil towards was read across from a study on orange oil according to OECD guideline 203 under GLP. Fish were exposed to nominal WAFs of 1.0, 2.0, 4.0, 8.0 and 10.0 mg/l in a semi-static setup and observed for 96 hours. The concentration of hydrocarbons was measured. Validity criteria for the test were met.
The acute toxicity (96h-EL50) of orange oil towards Danio rerio with a WAF test is 5.65 mg/l and the NOEL was 4.0 mg/L.
Supporting read across studies are also available for lime and mandarin oil. These studies report 96h-NOEL and 96h-LL50 values of 18 and >18 and 10 and >100 mg/l respectively.
The orange oil study was selected for read across, as the composition of orange oil closely resembles the composition of lemon oil and the EL50 is the lowest value.
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