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Toxicity to soil macroorganisms except arthropods

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Description of key information

NOEC on Eisenia fetida after the test period was determined to be >= 1000 mg/kg dry soil.

Key value for chemical safety assessment

Additional information

The toxic effects of the test item Amides, C16-C18 (even), N,N’-ethylenebis on survival, growth, and reproduction of the earthworm Eisenia fetida were assessed during a test period of eight weeks based on the OECD Guidelines for Testing Chemicals, Test No. 222 (April 2004) and the International Standards ISO 11268-2: 1998(E) (Eisner, 2014).

Amides, C16-C18 (even), N,N’-ethylenebis was homogeneously mixed into artificial soil at the following test concentrations:

62.5, 125, 250, 500, and 1000 mg test item/kg dry soil.

Additionally, a control treatment (substrate without test item) and a reference item treatment (Derosal®) were tested in parallel.

Adult earthworms were exposed in treated soils for a period of four weeks. After this period, the adults were removed from the test vessels and the survival and growth rate were determined. The cocoons and juvenile earthworms remained in the vessels for additional four weeks. The reproduction rate was determined by counting the number of offspring hatched from the cocoons after this additional test period of four weeks.

In conclusion, taking into account the survival, growth and reproduction rates of the exposed adult test organisms, the highest concentration of Amides, C16-C18 (even), N,N’-ethylenebis without toxic effects on Eisenia fetida (NOEC) after the test period was determined to be at least 1000 mg/kg dry soil. The NOEC might even be higher, but concentrations in excess of 1000 mg/kg dry soil were not tested. The lowest concentration with toxic effects (LOEC) could not be quantified, but was higher than 1000 mg/kg dry soil.