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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
Non-GLP, non-guideline animal experimental study, published in peer reviewed literature, limitations in reporting but otherwise adequate for assessment

Data source

Reference
Reference Type:
publication
Title:
Self-catalysed inactivation of hepatic cytochrome P-450 by ethynyl substrates
Author:
Ortiz de Montellano, P.R., and Kunze, K.L.
Year:
1980
Bibliographic source:
J. Biol. Chem., 255, 5578-5585

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Acetylene gas was administered by placing phenobarbital-pretreated rats for 4 h in a chamber through which a stream of air containing 10 to 15% (v/v) (equivalent to 107,000 - 160,500 mg/m3). acetylene was passed at an approximate rate of 200 to 300 ml/min. The interaction of acetylene with hepatic P-450 was investigated in-vitro and in-vivo.
GLP compliance:
not specified
Test type:
other: in-vivo and in-vitro
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Acetylene
EC Number:
200-816-9
EC Name:
Acetylene
Cas Number:
74-86-2
Molecular formula:
C2H2
IUPAC Name:
acetylene
Constituent 2
Reference substance name:
74-86-5
IUPAC Name:
74-86-5
Details on test material:
- Acetylene obtained from Matheson Chemical Co.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male

Administration / exposure

Route of administration:
inhalation: gas
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
INHALATION EXPOSURE:
- Animals were pre-treated for 4 days with 80 mg/kg/day sodium phenobarbital in aqueous solution by intraperitoneal injection
- Pre-treated rats were exposed in a chamber through which was passed the test atmosphere at a rate of 200-300 mL/min
Duration of exposure:
4 h
Concentrations:
10-15% v/v acetylene in air (equivalent to 107,000 - 160,500 mg/m3)
No. of animals per sex per dose:
no data

Results and discussion

Effect levels
Key result
Sex:
male
Dose descriptor:
LC0
Effect level:
160 500 mg/m³ air (nominal)
Based on:
test mat.
Exp. duration:
4 h
Mortality:
no mortality

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Acute inhalation exposure of male Sprague Dawley rats to 10-15% v/v acetylene (equivalent to 107,000 - 160,500 mg/m3) for 4 hours did not result in mortality.
Executive summary:

In an acute inhalation toxicity study, groups of male Sprague Dawley rats were exposed by inhalation route to acetylene in air for 4 hours to whole body at concentrations of 10-15% (v/v) acetylene in air (equivalent to 107,000 - 160,500 mg/m3).

There was no mortality observed after 4 hours exposure.

Based on these results, GHS criteria are not met and no classification according to Regulation (EC) No. 1272/2008 is warranted.