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Short-term toxicity to aquatic invertebrates

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In a key static 48-hour short-term Daphnia magna toxicity test (OECD 202; KS = 2), 10 animals/loading were exposed to the Water Accommodated Fraction of an other lubricant base oil, MVI(N) 40 base oil (CAS # 64742-53-6 or 64741-97-5), at nominal concentrations of 0, 10, 100, 1000, and 10,000 mg/L. The EL50 was >10,000 mg/L based on mobility and the NOEL was ≥ 1000 mg/L (Shell, 1988). 

 

In a key semi-static 96-hour short-term freshwater shrimp (Gammarus pulex) toxicity test (OECD 202; KS = 2), 10 animals/loading were exposed to the WAF of an other lubricant base oil, MVI(N) 40 base oil (CAS # 64742-53-6 or 64741-97-5), at nominal concentrations 0, 10, 100, 1000, and 10,000 mg/L. The LL50 was >10,000 mg/L and the NOEL was ≥ 10,000 mg/L (Shell, 1988).

 

These studies are scientifically sound and satisfy the guideline requirements for short-term invertebrate tests.