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Diss Factsheets

Administrative data

Endpoint:
biotransformation and kinetics
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2020

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
- Principle of test: Quantification of metabolites formed during degradation of 2-butoxyethanol
- Short description of test conditions: 3hrs exposure, then collection of metabolites by solvent extraction
- Parameters analysed / observed: Analysis of metabolites by GC-MS.
GLP compliance:
not specified
Type of medium:
aquatic

Test material

Constituent 1
Chemical structure
Reference substance name:
2-butoxyethanol
EC Number:
203-905-0
EC Name:
2-butoxyethanol
Cas Number:
111-76-2
Molecular formula:
C6H14O2
IUPAC Name:
2-butoxyethanol

Results and discussion

Transformation products:
yes
Identity of transformation productsopen allclose all
No.:
#1
Reference
Reference substance name:
Unnamed
IUPAC name:
butoxyacetic acid
No.:
#2
Reference
Reference substance name:
Unnamed
IUPAC name:
butan-1-ol
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 200-751-6]
CAS number:
71-36-3
Identity:
1-Butanol
Molecular formula:
C4H10O
Molecular weight:
74.122
SMILES notation:
CCCCO
InChl:
InChI=1/C4H10O/c1-2-3-4-5/h5H,2-4H2,1H3
No.:
#3
Reference
Reference substance name:
Unnamed
IUPAC name:
sodium butyrate
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 205-857-6]
CAS number:
156-54-7
Identity:
Butanoic acid, sodium salt
Molecular formula:
C4H8O2.Na
Molecular weight:
110.087
SMILES notation:
[Na+].CCCC(=O)[O-]
InChl:
InChI=1/C4H8O2.Na/c1-2-3-4(5)6;/h2-3H2,1H3,(H,5,6);/q;+1/p-1

Any other information on results incl. tables

As well as the transformation products, unchanged 2 -butoxyethanol was also detected.

This study was part of a larger study that examined the potential of 11 bacterial strains to degrade 2 -butoxyethanol. This particular species/strain grew the fastest and tolerated the highest substrate concentrations among the strains/species examined (and hence why it was used for this metabolite study). The maximum growth rate was 0.645 h-1 at a concentration of 5 mM (590mg/L), which was completely degraded within less than 6 h. A stable degradation of 2-BE could be achieved for concentrations of up to 30 mM (3540mg/L). Beyond that value, 2 -butoxyacetic acid

accumulated and the pH decreased to 6, inhibiting further growth.

.

Applicant's summary and conclusion

Executive summary:

In a study that qualitatively measured the metabolites produced by bacterial degradation of 2 -butoxethanol, the bacterial strain Pseudomonas vancouverensis was found to produce the metabolites 2 -butoxyacetic acid, butanol and butanoic acid. These findings support the hypothesis that 2 -butoxyethanol degradation by bacteria in the environment proceeds by initial oxidation to 2 -butoxyacetic acid (as in mammals) followed by cleavage of the ether group to produce butanol and butanoic acid. The maximum growth rate was 0.645 h-1 at a concentration of 5 mM (590mg/L), which was completely degraded within less than 6 h. A stable degradation of 2-BE could be achieved for concentrations of up to 30 mM (3540mg/L).

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