12-hydroxystearic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

From May 22, 1989 to May 26, 1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to accepted guidelines in compliance with GLP.

Justification for data waiving:

other:

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: ISO-guideline 7346/2

Deviations:

no

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Castor oil, hydrogenated

EC Number:

232-292-2

EC Name:

Castor oil, hydrogenated

Cas Number:

8001-78-3

IUPAC Name:

8001-78-3

Details on test material:

- Name of test material (as cited in study report): Castor oil, hydrogenated
- Analytical purity: 100%

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct weighing of the test substance into the test vessels, fill up with standard dilution water, then stirring 5 min by ultraturax.
- Eluate: Water

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Feeding during test: No

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

250 mg CaCO3/L

Test temperature:

22.5 - 23.5°C

pH:

7.6 - 8

Dissolved oxygen:

68 - 97%

Nominal and measured concentrations:

0, 1,000, 3,000 and 10,000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Fish basins
- Material, size, headspace, fill volume: 10 L
- Aeration: Oxygen saturated
- Renewal rate of test solution (frequency/flow rate): Daily
- Number of fish per concentration: 10

OTHER TEST CONDITIONS
- Photoperiod: 16 h illumination per d

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality

TEST CONCENTRATIONS
- Test concentrations: 0, 1,000, 2,000 and 10,000 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: None

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: None

Any other information on results incl. tables

Sublethal observations / clinical signs:

None.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the study, the 96h LC50 of 12-hydroxystearic acid to Zebra fish (Brachydanio rerio) was determined to be > 1000 mg/L.

Executive summary:

A study was conducted to determine the acute toxicity of the test substance to Zebra fish (Brachydanio rerio), in accordance with ISO-Guideline 7346/2 which conforms to the OECD Guideline 203 and in compliance with GLP.

The substance was tested in a concentration range of 1,000 - 10,000 mg/L and the incubation period was 96 h under semi-static conditions.

Under the conditions of the study, the 96h LC50 of test substance to Zebra fish was determined to be > 1,000 mg/L.