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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1998-07-14 to 1998-07-16
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
Method of preparation
Preparation of a turbid stock solution with a nominal concentration of 1 g product/1000 ml M4 media (synthetic media, M4-Medium according to Elendt). Pipetting of appropriate aliquots (permanent stirring) to test vessels. Subsequently the test vessels were filled up to 40 ml with M4-media.
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
- no data
Test temperature:
20.2 - 20.3 °C
pH:
7.5 - 7.8 after 48 h
Dissolved oxygen:
86 - 95 % O2 after 48 h
Salinity:
-
Nominal and measured concentrations:
Nominal concentrations: control, 1, 2.2, 4.84, 10.6, 23.4, 51.5, 113 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: closed by watch glass
- Material, size, headspace, fill volume: 100 ml beakers with watch glas, 50 ml fill volume
- Aeration: no data
- No. of organisms per vessel: no data, but suitable number acording t EU method C.2 is assumed
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium according to Elendt

OTHER TEST CONDITIONS
- Photoperiod: 16 h daily
- Light intensity: ca. 900 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobilisation


Reference substance (positive control):
yes
Remarks:
K2Cr2O4
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.23 mg/L
Nominal / measured:
estimated
Conc. based on:
other: corrected for recovery of 93%
Basis for effect:
other: immobilisation
Remarks on result:
other: a.i. 100 %
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
ca. 0.93 mg/L
Nominal / measured:
estimated
Conc. based on:
other: corrected for rec overy of 93%
Basis for effect:
other: immobilisation
Remarks on result:
other: a.i. 100 %
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
ca. 4.46 mg/L
Nominal / measured:
estimated
Conc. based on:
other: corrected for recovery of 93%
Basis for effect:
other: immobilisation
Remarks on result:
other: a.i. 100 %
Details on results:
Results of estimated effect concentration (basis for effect: immobilisation):
48 h EC50: 2.23 mg/L, corrected for recovery of 93%
48 h EC0: ca. 0.93 mg/L, corrected for rec overy of 93%
48 h EC100: ca. 4.46 mg/L, corrected for recovery of 93%

Results of nominal effect concentration (basis for effect: immobilisation):
48 h EC50: 2.4 mg/L, test mat., recovery in average 93 %
48 h EC0: 1 mg/L, test mat., recovery in average 93 %
48 h EC100: 4.8 mg/L, test mat.,recovery in average 93 %

- The average recovery rate was 93% of nominal concentration.
- No abnormal behaviour during test.
- Slight turbidity was observed at the two hightest test concentration (51.5, 113 mg/l)
Results with reference substance (positive control):
- Results with reference substance valid? yes
- EC50: 0.98 mg/l
Reported statistics and error estimates:
Calculation of EC50 resulted from semi-logarithmic interpolation.

Table 1: Percent Immobilisation of test organisms:

 

Concentration [mg/L]

Parallels

Immobilised Daphnia

After 24 h

After

0

1

0

0

 

2

0

0

1

1

0

0

 

2

0

0

2.2

1

10

50

 

2

20

40

4.84

1

90

100

 

2

80

100

10.6

1

100

100

 

2

100

100

23.4

1

100

100

 

2

100

100

51.5

1

100

100

 

2

100

100

113

1

100

100

 

2

100

100

 

Daphnids showed througout the test no abnormal behaviour.

At the two highest conventrations slight turbidity of the test solutiuons was observed

 

Table 2: DOC Analysis

 

Nominal concentration

Measured concentration

mg test mat./L

mg TOC/L

mg TOC/L

mg TOC/L

mg TOC/L

0 h

24 h

48 h

10.6

6,84

7.00

5.50

5.60

113

72.89

73.4

72.4

68.1

 

Table 3: Recovery of test material

 

Nominal concentration

% Recovery

% Mean recovery

 

mg test mat./L

mg TOC/L

0 h

24 h

 48h

10.6

6,84

102.4

80.4

81.9

88.2

113

72.89

100.7

99.3

93.4

97.8

 

The mean recovery of the test material was found to be 93% of nominal concentration.

 

Validity criteria fulfilled:
yes
Conclusions:
In this study the 48 h EC 50 of the test substance partially unsaturated TEA-Esterquat (solvent free) for Daphnia magna was 2.23 mg/l based on estimated concentration, corrected for recovery of 93% . The recovery rate was > 80 % of nominal concentration throughout the whole test.
Executive summary:

The 48 –hr-acute toxicity of partially unsaturated TEA-Esterquat (solvent free) to water flea (Daphnia magna) according to EU Method C.2 (Acute Toxicity for Daphnia) was studied under static conditions.  Daphnids were exposed to control, and partially unsaturated TEA-Esterquat (solvent free) at nominal concentration of 1, 2.2, 4.84, 10.6, 23.4, 51.5, 113 mg test mat./L for 48 hr.  Mortality/immobilization was observed daily. The 48 – hour EC50 was 2.23 mg a.i./L. The effect concentration was corrected for the analytical recovery of 93%. This study is classified as reliable without restriction and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.

 

Result synopsis 

Test Organism Age: Daphnia magna, 1st instar

Test Type: Static: EU Method C.2 (Acute Toxicity for Daphnia)

EC50:  2.23 mg a.i./L

Endpoint(s) Effected: mobility

Description of key information

48 h EC50 = 2.23 mg a.i./L (EU method C.2, Daphnia magna); GLP; RL1

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
2.23 mg/L

Additional information

Reliable and relevant data are available for partially unsaturated TEA-Esterquat.

 

The 48 hr acute toxicity of partially unsaturated TEA-Esterquat (solvent free) to water flea (Daphnia magna) according to EU Method C.2 (Acute Toxicity for Daphnia) was studied under static conditions.  Daphnids were exposed to control, and partially unsaturated TEA-Esterquat (solvent free) at nominal concentration of 1, 2.2, 4.84, 10.6, 23.4, 51.5, 113 mg test mat./L for 48 hr.  Mortality/immobilization was observed daily. The 48 hour EC50 was 2.23 mg a.i./L. The effect concentration was corrected for the analytical recovery of 93%.

 

In a supporting study, the 48 hr acute toxicity of partially unsaturated TEA-Esterquat (CAS No. 157905-74-3) was investigated in a study conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Under static conditions Daphnia magna were exposed to control and test chemical at nominal concentration of 0.2, 0.44, 0.97, 2.13, 4.69, 10.3, 22.7 mg/L (mean measured concentration: 0.09, 0.2, 0.43, 0.95, 2.1, 4.6, 10.1 mg/L). The mean concentrations during the test (mean of samples taken at 0 h and 48 h) were between 34.7 % and 51.7 % of nominal (average 44.5 %). Immobilization and sublethal effects were observed daily. The 48 hour EC50 based on mean measured concentrations was 6.05 mg/L.

 

The lowest 48 h EC50 in Daphnia was 2.23 mg a.i./L.