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Biodegradation in water and sediment: simulation tests

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Reference
Endpoint:
biodegradation in water: sewage treatment simulation testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06.10.1989 to 19.12.1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Qualifier:
according to guideline
Guideline:
OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units)
GLP compliance:
yes
Radiolabelling:
no
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge was collected from the aeration tank of a waste water treatment plant treating predominantly municipal sewage. The inoculum was sampled on October 5th, 1989.
- Preparation of inoculum for exposure: The activated sludge was aerated for 24 h before use.
- Pretreatment: no
- Concentration of sludge: not reported
Duration of test (contact time):
23 d
Initial conc.:
20 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Volume of test solution/treatment: 1 L
- Composition of medium: mineral medium was composed of peptone (160 mg/L), meat extract (110 mg/L), urea (30 mg/L), K2HPO4 (28 mg/L), NaCl (7 mg/L), CaCl2 x 2 H20 (4 mg/L), and MgSO4 x 7 H2O (2 mg/L) and was dissolved in deionized, air-saturated water.
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 21 +/- 1°C
- pH: pH measurements were conducted; the values, however, were not reported
- pH adjusted: not reported
- Aeration of dilution water: yes
- Suspended solids concentration: not reported
- Continuous darkness: yes
- Any indication of the test material adsorbing to the walls of the test apparatus: not reported

TEST SYSTEM
- Culturing apparatus: 2 laboratory waste water treatment plants (one receiving test material, one did not receive test material (control); the units were not coupled).
- Number of culture flasks/concentration: 1
- Method used to create aerobic conditions: Aeration of activated sludge before use; dilution water was oxygen-saturated
- Measuring equipment: DOC was measured (no further details reported)
- Test performed in open system: yes

SAMPLING
- Sampling frequency: sampling was performed on day 1, 2, 3, 4, 5, 8, 9, 10, 11, 12, 15, 16, 17, 18, and 23
- Sampling method: DOC was measured in the influent and effluent of the model waste water treatment plant
- Sample storage before analysis: no

CONTROL AND BLANK SYSTEM
One model waste water treatment plant which did not receive the test material served as control
% Degr.:
84.9
St. dev.:
6.52
Parameter:
DOC removal
Sampling time:
23 d
Transformation products:
not specified

The degradation of partially unsaturated TEA-Esterquat was investigated in a study conducted according to OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units). In laboratory waste water treatment plant, the test substance was biodegraded by 84.9% on average during 23 d.

Validity criteria fulfilled:
not specified
Conclusions:
The degradation of partially unsaturated TEA-Esterquat was investigated in a study conducted according to OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units). In laboratory waste water treatment plant, the test substance was biodegraded by 84.9% on average during 23 d.
Executive summary:

The aerobic biodegradation of partially unsaturated TEA-Esterquat was studied in a test according to OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units) and in compliance with the – GLP standards. Activated sludge was collected from the aeration tank of a waste water treatment plant treating predominantly municipal sewage. The test system consisted of 2 laboratory waste water treatment plants (one receiving test material, one did not receive test material (control); the units were not coupled). After periods of stabilizing and adaptation; increasing amounts of partially unsaturated TEA-Esterquat were introduced into the system: 2.5 mg to 20 mg/L DOC. Degradation was followed by DOC measurements in the influent and effluent of the model waste water treatment plant.  Samples were analyzed at day 1, 2, 3, 4, 5, 8, 9, 10, 11, 12, 15, 16, 17, 18, and 23.  The test substance was biodegraded by 84.9% on average during 23 d.

Description of key information

Key value for chemical safety assessment

Additional information

The aerobic biodegradation of partially unsaturated TEA-Esterquat was studied in a test according to OECD Guideline 303 A (Simulation Test - Aerobic Sewage Treatment. A: Activated Sludge Units) and in compliance with the – GLP standards. Activated sludge was collected from the aeration tank of a waste water treatment plant treating predominantly municipal sewage. The test system consisted of 2 laboratory waste water treatment plants (one receiving test material, one did not receive test material (control); the units were not coupled). After periods of stabilizing and adaptation; increasing amounts of partially unsaturated TEA-Esterquat were introduced into the system: 2.5 mg to 20 mg/L DOC. Degradation was followed by DOC measurements in the influent and effluent of the model waste water treatment plant.  Samples were analyzed at day 1, 2, 3, 4, 5, 8, 9, 10, 11, 12, 15, 16, 17, 18, and 23.  The test substance was biodegraded by 84.9% on average during 23 d.

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