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Short-term toxicity to aquatic invertebrates

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Short-term acute toxicity studies with three different species of invertebrates have been conducted and each study resulted in no observable effect concentration that was greater than the water solubility of the test substance. A pre-GLP 96-hour acute toxicity test was conducted with planorbid snails (H. trivolvis) at nominal concentrations up to 984 mg/L under static conditions. There was no mortality or abnormal effects observed at 984 mg/L (NOEC ≥984 mg/L).  A 48-h static acute OECD 202 toxicity limit test conducted with Daphnia magna under GLP resulted in a NOEC of ≥ 1.4ug/L.   Eastern oysters (C. virginica) in a 96-h acute toxicity study, were exposed at measured concentrations of 0, 30.3, 168, and 624 µg/L under flow-through conditions. There was no mortality or inhibition in shell deposition observed at any of the exposure concentrations during this study.  The 96-h NOEC value was ≥ 624 µg/L, the highest concentration tested.