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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
data is from peer reviewed journals

Data source

Reference
Reference Type:
publication
Title:
Toxicologic Studies on Brighteners
Author:
Snyder et.al
Year:
1963
Bibliographic source:
Toxicology and Applied Pharmacology, 1963

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To determine the most non-toxic dose of the test chemical when dosed orally to rats
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
- Name of test material (as cited in study report): Benzenesulfonic acid,2-2’-(1,2-ethenediyl)bis(5-((4-(bis(2- hydroxyethyl)amino)-6-(phenylamino)-1,3,5-triazin-2-yl)amino)-, disodium salt
- Molecular formula: C40H42N12O10S2.2Na
- Molecular weight : 960.9578 g/mole
- Substance type: Organic
- Physical State: Solid

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified
Details on test animals and environmental conditions:
no data available

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 20% suspension in water
Doses:
4.7-10 g/kg
No. of animals per sex per dose:
18 rats
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days (or other?): no data available
- Frequency of observations and weighing: no data available
- Necropsy of survivors performed: yes/no: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: mortality
Statistics:
no data available

Results and discussion

Preliminary study:
no data available
Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 10 000 mg/kg bw
Based on:
test mat.
Mortality:
No mortality was observed at the highest dose tested (10000 mg/kg)
Clinical signs:
no data available
Body weight:
no data available
Gross pathology:
no data available
Other findings:
no data available

Any other information on results incl. tables

Acute toxicity of brightners        

Species

Vehicle

Concentration (%)

Range of doses (g/kg)

Number of dose levels

Total number of animals

LD50

(g/kg)

Rat

Water

20

4.7-10.0

3

18

>10

Applicant's summary and conclusion

Interpretation of results:
other: Not Classified
Conclusions:
No mortality was observed at the highest dose tested, the acute oral LD50 of the test chemical can be considered to be greater than 10000mg/kg in rats.Hence, the test chemical cannot have any significant toxic effect in case of accidental ingestion.
Executive summary:

A study was performed to determine the most non-toxic dose of the test chemical when dosed orally to rats.18 rats were dosed with 20% suspension of the test chemical in water via stomach tube. The dose levels were in the range 4.7 -10.0 g/kg. The treated rats were observed for mortality(duration not mentioned).Since no mortality was observed at the highest dose tested, the acute oral LD50 of the test chemical can be considered to be greater than 10000mg/kg in rats.Hence, the test chemical cannot have any significant toxic effect in case of accidental ingestion.