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All three acute trophic levels were assessed either with study on the substance itself or with reliable Read Across studies on analogous substances. EC50 was always measured as higher than 100 mg/l.

Regarding long term studies, a Daphnia magna reproductive study is available on the similar substance 16470-24-9. The substance is the analogous substance dihydroxyethylamino derivative tetrasulphonated sodium salt. The result of the chronic reproductive Daphnia study on the analogous substance CAS 16470-24-9 has been considered to establish the NOEC for the substance (NOEC = 6.59 mg/l).

No further long term studies are proposed, since direct and indirect exposure is unlikely due to the risk management measures that will be applied at industrial level and discussed in details within the exposure scenario to avoid any release of the substance directly in the municipal waste water treatment. The local RCR in surface water is calculated as 0.044 and 0.0052 based on estimated and monitored quantities, respectively.