Registration Dossier
Registration Dossier
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EC number: 239-268-0 | CAS number: 15214-89-8
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented report of a guideline study conducted according to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EPA-66013-75-009, "methods for acute toxicity tests with fish, macrovertebrates, and amphibians.
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-acrylamido-2-methylpropanesulphonic acid
- EC Number:
- 239-268-0
- EC Name:
- 2-acrylamido-2-methylpropanesulphonic acid
- Cas Number:
- 15214-89-8
- Molecular formula:
- C7H13NO4S
- IUPAC Name:
- (1Z)-N-(2-methyl-1-sulfopropan-2-yl)prop-2-enimidic acid
- Details on test material:
- - Name of test material (as cited in study report): OS #26935F
- Substance type: organic
- Physical state: solid
- Analytical purity: not specified
- Impurities (identity and concentrations): not specified
- Composition of test material, percentage of components: 100%
- Purity test date: not specified
- Lot/batch No.: not specified
- Expiration date of the lot/batch: not specified
- Stability under test conditions: stable
- Storage condition of test material: not specified
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: dilution
- Chemical name of vehicle: soft water reconstituted from deionized water
- stock solution: 150 mg/mL
- final test solutions: 130, 220, 360, 600 and 1000 mg/L
Test organisms
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- TEST ORGANISM
- Common name: Bluegill
- Source: in house laboratory supply (Lot # 83A13)
- Age at study initiation: juvenile
- Length at study initiation: 3.8 cm
- Weight at study initiation: 0.66 g (wet weight)
- Feeding during test: no feeding during the study
ACCLIMATION
- Acclimation period: 14 days prior to start the test
- Acclimation conditions: same as test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 44 mg/L as CaCO3
- Test temperature:
- 22°C +/- 1
- pH:
- 7.5
- Dissolved oxygen:
- 5.5 to 9.2 mg 02/L
- Nominal and measured concentrations:
- Nominal concentration:
Control, 130; 220; 360; 600 andd 1000 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: glass exposure vessels containing 15 L of test solution
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): no replicates
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: soft water reconstituted from dionized water
- Alkalinity: 32 mg/L
- Conductivity: 140 microhos/cm
OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light and 8 hours darkness
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.6 - 1.7 - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 130 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 170 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 130 - 220
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 170 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 130 - 220
- Details on results:
- - Abnormal responses: sublethal effects including loss of equilibrium and rapid respiration was seen at 0 hours in the 600 and 1000 mg/L test concentration
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC0 at 96 hours for fish of ATBS was 130 mg/L and the LC50 at both 72 and 96 hours was calculated as 170 mg/L.
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