Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 253-575-7 | CAS number: 37640-57-6
Toxic effects on livestock and pets
Administrative data
- Endpoint:
- toxic effects on livestock and pets
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Adequate report of non-standard investigation
Data source
Reference
- Reference Type:
- publication
- Title:
- Identification and Characterization of Toxicity of Contaminants in Pet Food Leading to an Outbreak of Renal Toxicity in Cats and Dogs
- Author:
- Dobson, RLM et al
- Year:
- 2 008
- Bibliographic source:
- TOXICOLOGICAL SCIENCES 106(1), 251–262
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Research relating to the major recall of pet food that occurred in Spring 2007 in North America
- GLP compliance:
- no
- Limit test:
- no
Test material
- Details on test material:
- Melamine and cyanuric acid were obtained from Sigma-
Aldrich (Milwaukee, WI).
Constituent 1
Test animals
- Species:
- other: rats, cats and dogs
- Details on test animals and environmental conditions:
- not available
Administration / exposure
- Route of exposure:
- oral: applied on feed
- Vehicle:
- unchanged (no vehicle)
Results and discussion
- Details on results:
- Clinical observations of acute renal failure in cats and dogs were associated with consumption of wet pet food produced by
a contract manufacturer producing for a large number of companies. The affected lots of food had been formulated with wheat gluten originating from China. Pet food and gluten were analyzed for contaminants using several configurations of highperformance liquid chromatography (HPLC) and mass spectrometry (MS), which revealed a number of simple triazine compounds, principally melamine and cyanuric acid, with lower concentrations of ammeline, ammelide, ureidomelamine, and N-methylmelamine.
Infrared microspectroscopy on individual crystals from rat or cat (donated material from a veterinary clinic) kidneys confirmed that they were melamine-cyanuric acid cocrystals.
Rats given both melamine and cyanurate, but not melamine or cyanurate alone, showed crystals in the kidney upon three day dosing.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
