Registration Dossier

Administrative data

Endpoint:
immunotoxicity
Remarks:
other: mitogenesis test
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Scientific publication with appropriate description of the method.

Data source

Reference
Reference Type:
publication
Title:
Immunotoxicological Evaluation of Environmental Chemicals Utilizing Mouse Lymphocyte Mitogenesis Test
Author:
Sakazaki H, Ueno H, Umetani K, Utsumi H, Nakamuro K
Year:
2001
Bibliographic source:
J. Health Sci., Vol. 47(3), 258-271, 2001

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The aim of this study was to assess the immunotoxicity of environmentally relevant chemicals including melamine by means of an in vitro lymphocyte mitogenesis test. The growth-inhibitory effect of environmental chemicals was determined on the mouse splenic lymphocyte mitogenesis using lipopolysaccharide and concanavalin A as the specific mitogens for B cells and T cells. The toxicity on humoral immunity and cell-mediated immunity was evaluated, respectively.
When lymphocytes are stimulated by a mitogen, the following reactions occur on the lymphocytes: capping of the receptors or immunoglobulins on the surface of lymphocytes, increase of intracellular calcium concentration, activation of intracellular signaling pathways, phosphorylation and activation of each enzyme, synthesis of RNA and proteins, replication of DNA, and finally mitosis of lymphocytes. If lymphocytes have been exposed to an environmental chemical during the period of the mitogenesis, any inhibition in the sequential reactions can be detected as depression of lymphocyte proliferation. Therefore, the lymphocyte mitogenesis test may be useful for assessing immunotoxicity of environmental chemicals.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
melamine

Test animals

Species:
mouse
Strain:
other: C3H/He and BALB/c
Sex:
male

Administration / exposure

Route of administration:
other: in vitro assay
Duration of treatment / exposure:
Exposure period: 4 day(s)
Control animals:
yes
Details on study design:
Mouse Lymphocyte Mitogenesis Test

Results and discussion

Any other information on results incl. tables

Melamine did not inhibit the mitogenesis of B- and T-lymphocytes.

Applicant's summary and conclusion

Conclusions:
Melamine did not inhibit the mitogenesis of B- and T- lymphocytes.
Executive summary:

Melamine did not inhibit the mitogenesis of B- and T-lymphocytes.