Registration Dossier

Administrative data

Endpoint:
toxicity to other aquatic vertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study according to scientific standards, basic data given

Data source

Reference
Reference Type:
publication
Title:
Chronic Toxicity of Phenanthrene to the Marine Polychaete Worm, Nereis (Neanthes) arenaceodentata
Author:
Emery VL, Jr. and Dillon TM
Year:
1996
Bibliographic source:
Bull. Environ. Contam. Toxicol. 56, 265-270, 1996

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Emerging juvenile (EJ) polychaete worms were exposed to a concentration of 20 µg/L phenanthrene for 8 weeks in saltwater. Survival, growth, fecundity, EJ production, EJ size, and time from pairing to egg deposition were observed and evaluated. 96 h und 14 d LC50 were determined for emerging juveniles and immature adults respectively.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: solid
Details on test material:
- Name of test material (as cited in study report): phenanthrene (obtained from Aldrich Chemical Company, Milwaukee, Wisconsin, USA)
- Substance type: organic, PAH
- Physical state: solid
- Analytical purity: 98%
- Impurities (identity and concentrations):
- Lot/batch No.: Lot #01128E2

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance was introduced in conjunction with the normal worm feeding ration.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
- Concentration of vehicle in test medium (stock solution and final test solution): 1000 µg/L (immature adult test) and 100 µg/L (emerging juvenile test - EJ)
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no data

Test organisms

Test organisms (species):
other: Nereis (Neanthes) arenaceodentata
Details on test organisms:
TEST ORGANISM
- Common name: polychaete worm
- Age at study initiation (mean and range, SD): immature adult and emerging juvenils
- Feeding during test : yes
- Food type: TetraMarin-alfalfa food slurry
- Amount:
- Frequency: three times per week for adults test; once at the beginning of the emerging juvenil test

Study design

Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
8 wk

Test conditions

Salinity:
30 ‰
Details on test conditions:
TEST SYSTEM
- Test vessel: 38 L aquaria (8 wks chronic study), 250 mL beakers (EJ range finder test), 1 L beakers (immature adults range finder test)
- Type (delete if not applicable): open
- Material, fill volume: glas, 30 L (8 wks chronic study), 200 mL (EJ range finder test), 800 mL (immature adults range finder test)
- Aeration: no data
- Renewal rate of test solution (frequency/flow rate): no data
- No. of organisms per vessel: 100 (8 wks chronic study), 10 (EJ range finder test), 5 (immature adults range finder test)
- No. of vessels per concentration (replicates): 4 (8 wks chronic study), 3 (EJ range finder test), 4 (immature adults range finder test)
- No. of vessels per control (replicates): 4 (8 wks chronic study), 3 (EJ range finder test), 4 (immature adults range finder test)
- No. of vessels per vehicle control (replicates): 4 (8 wks chronic study), 3 (EJ range finder test), 4 (immature adults range finder test)
- Biomass loading rate: no data

TEST MEDIUM / WATER PARAMETERS
- No data

OTHER TEST CONDITIONS
- No data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : growth (dry weight), effects on reproduction (fecunditiy and EJ production), EJ size, time from pairing to egg depositions, total egg lipids, phenanthrene tissue content (chronic test), morftalitiy (EJ and immature adults range finder test

TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: 1 concentration for chronic study (no guideline study)
- Range finding study: 2 experiments, one with emerging juveniles, one with immature adults
- Test concentrations: 20 µg/L (chronic study); 10, 18, 32, 56, 100 µg/L (EJ range finder test); 100, 178, 316, 562, 1000 µg/L (immature adult range finding test
- Results used to determine the conditions for the definitive study: emerging juvenile 96-hr LC50 of 51 µg/L, immature adult 14-d LC50 of 501 µg/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
8 wk
Dose descriptor:
LOEC
Effect conc.:
20 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: growth, fecundity, emerging juveniles production
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
51 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL = 46-47 µg/L, test organism emergent juveniles
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
501 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL = 425-590 µg/L, test organism immature adults

Any other information on results incl. tables

There was no difference in LC50 results in experiments with and without sediment.

After 8 weeks of chronic exposure, survival was high and not significantly different among experimental treatments. The acetone carrier had a significant and positive effect on growth and reproduction relative to worms in the seawater control.

Compared to acetone carrier, treatment significantly diminished growth, fecundity and EJ production and significantly increased the time between pairing and egg deposition.

Worms accumulated 780 µg phenanthrene/kg ww during the 8 week exposure.

Exposed worms produced copious amounts of mucus and did not actively feed during the latter half of the 8 week exposure. When removed from the aquaria, they were very lethargic and did not exhibit the normal fighting behaviour. Normal behaviour resumed after 24 hrs in clean seawater.

Symptoms indicate non-specific narcosis.

Applicant's summary and conclusion