Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Currently viewing:

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The data are summarized in the peer-reviewed literature. The studies were not performed under Good Laboratory Practices nor according to a validated testing guideline; however, the data are well documented and scientifically acceptable.

Data source

Reference
Reference Type:
publication
Title:
Effects of Halogens on Algae-I. Chlorella sorokiniana
Author:
Kott, Y., and Edlis, J.
Year:
1969
Bibliographic source:
Water Res., Vol. 3, Iss. 4, pp. 251-256.

Materials and methods

Test guideline
Qualifier:
no guideline required
Principles of method if other than guideline:
No testing guideline was available when these studies were performed. Standard acute toxicity testing methods were employed.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Hypobromous acid, sodium salt
- Other: Bromine dissociates in aqueous solutions to hypobromous acid (Beckwith et al., 1996).

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Chlorella sp.
Details on test organisms:
Chlorella sorokiniana, 225 cells/mL

Study design

Test type:
other: Renewal and static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
20 h
Remarks on exposure duration:
An 18-h exposure was also performed.
Post exposure observation period:
Not reported

Test conditions

Hardness:
Not reported
Test temperature:
18 h exposure = 28-32 ºC
20 h exposure = 30 ºC
pH:
18 h and 20 h exposure = pH 7.0
Dissolved oxygen:
Not reported
Salinity:
Not reported
Nominal and measured concentrations:
18 h exposure = measured concentration of 1000 micrograms/L.
20 h exposure = measured concentrations with a mean value of 600 micrograms/L and a range from 200 to 600 micrograms/L.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
18 h
Dose descriptor:
other: Not reported
Effect conc.:
>= 1 000 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
cell number
Duration:
20 h
Dose descriptor:
other: Not reported
Effect conc.:
ca. 600 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: mortality
Remarks on result:
other: Renewel, range from 200 to 600 micrograms/L
Duration:
20 h
Dose descriptor:
other: Not reported
Effect conc.:
ca. 600 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
other: mortality
Remarks on result:
other: Static, range from 200 to 600 micrograms/L

Applicant's summary and conclusion

Conclusions:
An 18-hour effect concentration on population growth was reported at a concentration of 1000 micrograms/L. The 20-hour effect concentration was 600 micrograms/L under renewel and static conditions.