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Toxicity to microorganisms

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Description of key information

An ASRI study was performed with bentonite acid leached in accordance with OECD guideline 209 (Activated sludge, respiration inhibition test) The EC50 was > 1000 mg/L after 3 hours contact time.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
1 000 mg/L

Additional information

In a key study (Clarke 2010) activated sewage sludge was exposed to an aqueous dispersion of the test item at concentrations of 10, 32, 100, 320 and 1000 mg/L for a period of 3 hours at a temperature of 21 ± 1°C with the additiona of a synthetic sewage as a respiratory substrate. The rate of respiration was determined after 3 hours contact time and compared to data for the control and a reference item, 3,5 -dichlorophenol. The effect of the test item on the respiration of activated sewage sludge gave a 3 hour EC50 of > 1000 mg/L. The NOEC after 3 hours exposure was 1000 mg/L.