Toluene-4-sulphonic acid

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

other: Read across from an analogous substance

Adequacy of study:

key study

Study period:

1962

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1962

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

9 animals were exposed to 0.1 mL of the test substance for 5, 10 or 30 seconds (3 animals for each duration) and then eyes were rinsed. The Draize, Woodard and Calvery scoring system was used. Observations were at 1, 4, 24, 48, 72, 120 and 168 hours following exposure. Reversibility was determined up to 14 days post exposure.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-hydroxybenzenesulphonic acid

EC Number:

202-691-6

EC Name:

4-hydroxybenzenesulphonic acid

Cas Number:

98-67-9

Molecular formula:

C6H6O4S

IUPAC Name:

p-hydroxybenzenesulphonic acid

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not specified

Duration of treatment / exposure:

5, 10 or 30 seconds

Observation period (in vivo):

168 hours for scoring and 14 days for reversibility

Number of animals or in vitro replicates:

3 at each treatment duration

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 5 second exposure

Score:

>= 83.6 - < 110

Max. score:

110

Reversibility:

not fully reversible within: 14 days

Remarks on result:

other: overall irritation score was 83.6 out of 110 at 5 second exposure, and 110 out of 110 at both 10 and 30 second exposures

Other effects:

With 5 second exposure there was copious discharge, swelling with lids about half closed, severe redness of conjunctivae, and opacity sufficient to render the iris invisible, all developed within a few minutes. After 14 days there is moderate edema and discharge, moderately severe erythema, and opalescent areas. The iris is reacting to light.
With 10 second exposure, the response was similar to 5 seconds except the iris seems to be destroyed at 14 days. Maximum score of 110 reached in 4 hours.
With 30 second exposure, the response was similar to 10 seconds but with the area of severe edema and congestion extending for a greater distance around the eye.
The cornea became opalescent in 4-8 seconds and the swelling was rapid in all exposures.

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye) based on GHS criteria

Conclusions:

Eye Damage 1, H318: Causes serious eye damage.

Executive summary:

The eye irritation of Hydroxybenzene sulphonic acid was assessed with a test where 9 animals were exposed to 0.1 mL of the test substance for 5, 10 or 30 seconds (3 animals for each duration) and then eyes were rinsed. The Draize, Woodard and Calvery scoring system was used. Observations were at 1, 4, 24, 48, 72, 120 and 168 hours following exposure. Reversibility was determined up to 14 days post exposure. The test substance is corrosive to the eye even with short (i.e., 5-30 second) exposure.