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Toxicity to microorganisms

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Description of key information

EC50 > 10,000 mg/L for activated sludge (DIN 38412, part 8)

Key value for chemical safety assessment

Additional information

There are no studies available on the toxicity of Fatty acids, coco, 2-ethylhexyl esters (CAS No. 92044-87-6) to microorganisms. The assessment was therefore based on studies conducted with the structurally most similar category members, for which data is available, dodecanoic acid isooctyl ester (CAS No. 84713-06-4) and isopropyl myristate (CAS No. 110-27-0), as part of a read-across approach. This is in accordance with Regulation (EC) No 1907/2006, Annex XI, 1.5. Grouping of substance and read across approach. Further justification is given within the endpoint summary 6.1 and within the category justification Section 13. In this case of read-across, the best suited (highest degree of similarity, nearest physico-chemical properties) read-across substances were entered into IUCLID. Nevertheless, as can be seen in the data matrix of the category justification in Section 13, all reliable data in the category support the hazard assessment for this endpoint, by showing a consistent pattern of results.

The key study with dodecanoic acid isooctyl ester (CAS No. 84713-06-4) was performed according to the national guideline DIN 38412, part 8 (van Erp, 1997). A culture of the test organism Pseudomonas putida was exposed to the test substance in a static system for 16 hours, at nominal concentrations of 0, 310, 625, 1250, 2500, 5000 and 10,000 mg/L. No toxicity was observed at any of the concentrations, and the EC50 was reported to be > 10,000 mg/L.

The supporting study with isopropyl myristate (CAS No. 110-27-0) was performed according to a national guideline similar to DIN 38412, part 8 and GLP (Coenen, 1988). A culture of the test organism Pseudomonas putida was exposed to the test substance in a static system for 18 hours, at the nominal test concentrations 0, 0.004, 0.008, 0.016, 0.031, 0.063, 0.125, 0.25, 0.5, 1, 2, 4, 8, 16 mg/L. Since all the measured extinction values were very close to the reference, no real toxicity threshold could be determined. It can, however, be concluded that the substance has no effect on the growth of P. putida up to the concentration of 16 mg/L.

Thus, based on theabove mentioned result, and due to the structural and profile similarities of the two substances, as are explained with in the overall endpoint summary 6.1 it can be concluded that the EC50 (nominal) of Fatty acids, coco, 2-ethylhexyl esters (CAS No. 92044-87-6) is > 10,000 mg/L.