Tricobalt tetraoxide

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Comparable to guideline study with acceptable restrictions - The purity and stability of the test material were not stated. Minor deviations with no effect on the study results: -According to the guideline, the volume administered to the animals should not exceed 1ml/100 g bw. The information on the volume administered was missing in the study report. -According to the guideline, the diet and environmental conditions shoud be stated in the test report. This information was missing in this report.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Version / remarks:

1987-02-24

Deviations:

yes

Remarks:

, see "rational for reliability"

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tricobalt tetraoxide

EC Number:

215-157-2

EC Name:

Tricobalt tetraoxide

Cas Number:

1308-06-1

Molecular formula:

Co3O4

IUPAC Name:

dicobalt(3+) λ²-cobalt(2+) tetraoxidandiide

Details on test material:

- Name of test material (as cited in study report): Cobalt oxide tricobalt tetraoxide
- Batch No.: S90673
No further nformation on the test material was stated.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Breeding Laboratories, Inc., Wilmington, MA
- Weight at study initiation: males mean body weight (fasted): 309.2 +/- 14.4 g; females mean body weight (fasted): 210.8 +/- 11.7 g
- Fasting period before study: Food was withheld the night prior to dosing
- Housing: Individually housed in wire mesh bottom cages
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: At least 5 days
No further information on the test animals was stated.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

VEHICLE
The test item was suspended in corn oil. The concentration prepared was 50 %.
DOSAGE PREPARATION:
Weighed 20 gm test article, Q.S. to 40 ml with diluent.
No further information on the oral exposure was stated.

Doses:

5000 mg/kg

No. of animals per sex per dose:

5 males/5 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 15 days
- Frequency of observations and weighing: All animals were observed frequently on the day of dosing and twice daily for the remainder of the study. All external signs of toxicity or pharmacological effects were noted. Body weights were recorded initially, on days 8 and 15 or at death.
- Necropsy of survivors performed: Yes
All animals that died and those sacrificed at termination of the study were subjected to a gross necropsy and abnormalities were noted.
No further information on the study design was stated.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

All animals survived the 15 day post-dose observation period.

Clinical signs:

other: All animals appeared normal throughout the study.

Gross pathology:

No noteworthy findings were made at necropsy.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The LD50 (male and females rats) for Cobalt oxide tricobalt tetraoxide is:
LD50 (male and females rats) > 5000 mg/kg
According to the EC Regulation No. 1272/2008 and subsequent regulations, the test item is not classified.