1,6-Octadiene, 8-methoxy-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted in compliance to GLP following OECD-203 (Fish, Acute toxicity test) guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

Stated purity: 99.2%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

For formulation of the test concentrations used in the main study stock solutions of 500 and 280 mg/l test substance suspended in 2l of tapwater containing 0.2 ml Tween 80, were used. The suspensions formed were homogeneous and turbid. Subsequently the final test substance concentrations were made by diluting the stock solutions with tapwater.
The final volume was 10l.

CONTROLS:
Blank: test medium without test substance or other additives.
Tween-blank: test medium without test substance, but with the applied additive Tween 80, at a concentration of 0.02ml/l

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Length: 2-4 cm
Weight: 0.43 g (mean, n=10)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.33mmol/l

Test temperature:

18-24°C

pH:

8.0-8.6

Dissolved oxygen:

7.5-9.4mg/l

Nominal and measured concentrations:

Nominal: 0, 5.6, 10, 18, 32, 56 mg/l

Reference substance (positive control):

yes

Remarks:

A reference test with pentachlorophenol (PCP, SIGMA), carried out every 3 months.

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

24 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CI = 18-32mg/L

Details on results:

After 3 hours o f exposure all fish had died at 56 mg/l and 6 fish had died at 32 mg/l. The remaining 4 fish at 32 mg/l concentration were found immobile after 3 hours of exposure and had died after 24 hours of exposure. No mortality was observed at the other concentrations or the controls.

Effects other than mortality and immobility, were observed at 18 mg/l. These were: hypoactivity and discolouring. At 18 mg/l the number of affected fish and the type of effect remained constant after 24 hours.

Applicant's summary and conclusion

Conclusions:

96h-LC50 for fish exposed to 1-Methoxy-2,7-Octadiene is calculated to be nominally 24mg/l with 0% mortality at 18 mg/l and 100% mortality at 32 mg/l.