Diphenyl carbonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

Principles of method if other than guideline:

Directive EG L 133, part C

GLP compliance:

yes

Analytical monitoring:

no

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Concentration of the activated sludge was 320 mg/l

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test temperature:

20 ± 2 °C

pH:

6.8

Dissolved oxygen:

Oxygen consumption was recorded over a period of 6 minutes

Nominal and measured concentrations:

1000, 1800, 3200, 5600, 10000 mg/L (nominal)

Details on test conditions:

The system was aerated and stirred during the test

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

4 510 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

60 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

The EC50 was obtained with Probit Analysis whereby all concentrations were weighted equal.

Conclusions:

The 3h EC10 and the 3h EC50 of diphenyl carbonate to activated sludge is 60 mg/l and 4510 mg/l, respectively.

Executive summary:

The principles of the method are those of Directive EG L 133, part C and correspond to the standardised guideline OECD 209. The study was conducted under GLP conditions.

During the study domestic activated sludge was exposed to the test material for 3 hours under static conditions at nominal concentrations of 1000, 1800, 3200, 5600 and 10 000 mg/L. The concentration of the activated sludge was 320 mg/L.

The EC50 was obtained with Probit Analysis whereby all concentrations were weighted equal. This study identifies the 3 h EC10 and the 3 h EC50 of diphenyl carbonate to activated sludge as 60 and 4510 mg/L, respectively.

Description of key information

The toxicity of diphenyl carbonate to activated sludge was assessed using methodology comparable to OECD 209 (Bayer, 2000c). A 3 h EC10 of 60 mg/L and a 3 h EC50 of 4510 mg/L were determined.

Key value for chemical safety assessment

EC50 for microorganisms:

4 510 mg/L

EC10 or NOEC for microorganisms:

60 mg/L

Additional information

This endpoint is addressed with one key study and one supporting study.

 

In the key study (Bayer, 2000c) the principles of the method are those of Directive EG L 133, part C and correspond to the standardised guideline OECD 209. The study was conducted under GLP conditions and was awarded a reliability score of 1 in accordance with the criteria for assessing data quality as set forth by Klimisch et al. (1997).

Domestic activated sludge was exposed to the test material for 3 hours under static conditions at nominal concentrations of 1000, 1800, 3200, 5600 and 10 000 mg/L. The concentration of the activated sludge was 320 mg/L

The EC50 was obtained with Probit Analysis whereby all concentrations were weighted equal. This study identifies the 3 h EC10 and the 3 h EC50 of diphenyl carbonate to activated sludge as 60 and 4510 mg/L, respectively.

 

The supporting study (Bayer, 1979) has been awarded a reliability score of 4 in accordance with the criteria for assessing data quality as set forth by Klimisch et al. (1997) as the documentation is insufficient for assessment. The study was not performed under GLP conditions, but follows the methodology of the cell multiplication inhibition test according to Bringmann and Kuehn (1977). The study identifies the EC0 to Pseudomonas putida (after a testing period of 16 hours) as > 280 mg/L.