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EC number: 225-208-0 | CAS number: 4719-04-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
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- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Activated sludge from laboratory wastewater plant treating municipal sewage.
- The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated during the night.
- 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- 20+/-2°C
- Dissolved oxygen:
- - During aeration: >2.5 mg/L
- Immediately before measurement: >6.5 mg/L - Nominal and measured concentrations:
- - Nominal concentrations of test substance (mg/L): 10, 100, 248, 496, 1000
- Details on test conditions:
- - Test vessels: Erlenmeyer-vessels (nominal volume 250 mL)
- Test volume: 250 mL
- Synthetic medium: 8 mL/vessel 100-fold concentrated OECD - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 170 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 550 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - EC50 of 3,5-dichlorophenol in the range of 5-30 mg/L
- Deviation of blank controls < 15%
Reference
Description of key information
The test substance was tested in an OECD guideline study. The EC50 was determined to be 550 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 550 mg/L
Additional information
Freshwater
Key
The test substance was tested in an OECD guideline 209 study. The nominal concentrations tested were 10, 100, 248, 496, 1000 mg/L.The test was conducted under static conditions. Domestic activated sludge was used as inoculum. The total exposure duration was 30 min. The EC50 was determined to be 550 mg/L.
Supporting
The toxicity to microorganisms was tested in a study according to DIN 38412-8. The nominal concentration (mg/L) used were: 39.06, 78.13, 156.25, 312.5, 625, 1250, 2500, 5000, 10000. The EC50 value was determined to be 55.4 mg/L and the EC10 value was determined to be 41.3 mg/L. Both values based on growth inhibition.
The toxicity to microorganisms was tested in this OECD guideline study. The test substance was tested under static conditions with a total exposure duration of 30 min. The EC50 value was determined to be 560 mg/l.
The toxicity to microorganisms was tested in this OECD guideline study. Only few data were available for this study. The EC50 value was determined to be 370 mg/L and the EC10 value was determined to be 140 mg/L.
Conclusion: 4 different studies were available. 3 OECD guideline studies were available one study according to DIN 38412 -8. The results of the guideline studies lay all in the same range. As a result the EC50 value of 550 mg/L was chosen as key result.
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