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EC number: 225-208-0 | CAS number: 4719-04-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
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- Specific investigations
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- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 2005-10-19 to 2006-01-17
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The purity of the test substance is not indicated.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 306 (Biodegradability in Seawater)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- natural water: marine
- Details on inoculum:
- - Source of inoculum/activated sludge: Populations of bacteria which occur naturally in seawater. The raw seawater used for this study was supplied by a submersible pump situated on Sutherland's pier on the west side of Flotta in Scapa Flow. It is pumped continuously from a depth of two metres below low water spring tide level, before passing up 1.8 kilometres of plastic pipe to a 20,000 litre storage tank.
- Duration of test (contact time):
- 28 d
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: According to guideline
- Test temperature: 20 +/- 1 °C, seawater temperature: 6-14 °C in the summer
- Salinity: 34 and 37 %.
TEST SYSTEM
- Culturing apparatus: 272 mL glass BOD bottles
- Number of culture flasks/concentration: 3, minimum of 2 per timepoint for data processing
CONTROL AND BLANK SYSTEM
- Toxicity control: yes, to enable an assessment of potential inhibitory effects of the test material (or its primary degradation products), an inhibition control is used, in which a mixture of the soluble reference compound and the test material is tested.
STATISTICAL METHODS: The overall assessment of biodegradability is based upon a comparison between experimentally determined oxygen consumption (BOD measurements) and the oxygen consumption predicted if all carbon present in the test material were completely oxidised (theoretical oxygen demand, ThOD). - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- Not performed
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 88
- Sampling time:
- 28 d
- Details on results:
- The test was conducted in accordance with the study plan and met all relevant validity criteria. There were no interferences in this test. The test substance biodegraded by 88% over 28 days and showed an inhibition of 15% to seawater bacteria. The oxygen blank was within formal limits of acceptability.
- Results with reference substance:
- The soluble reference material, sodium benzoate, degraded by more than 60% in the first ten days, indicating that the seawater used in the test contained a satisfactory population of viable bacteria.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Duration of test (contact time):
- 8 d
- Initial conc.:
- 50.7 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- >= 90 - <= 100
- Sampling time:
- 8 d
- Details on results:
- lag phase: 1 day
start of plateau phase: day 3
Elimination by adsorption: less than 10% after 1 day.
Abiotic elimination: less than 10% after 8 days.
The reference substance (aniline) was functional. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
Referenceopen allclose all
Description of key information
Four readily biodegradable studies according to OECD criteria are available. Three studies were tested with freshwater one was tested with salt water. As a result the test substance is classified as readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Four studies performed with freshwater according to OECD guideline 301 and OECD guideline 310 are available. One further study performed with salt water according to OECD guideline 306 was performed.
Freshwater
Key
The readily biodegradability of the test substance was determined in a study according to OECD guideline 301 A. As inoculum activated sludge, domestic, non-adapted was used. The initial concentration was 50.7 mg/L test material and 20.0 mg/L DOC. Aniline was used as reference substance. A degradation of 90 – 100 % was determined after 8 days. The substance is ready biodegradable.
Supporting
The ready biodegradability of the test substance was determined in a study according to OECD guideline 301 C. Activated sludge was used as inoculum. An initial concentration of 100 mg/L was used. The test showed a degradation of 89.0 – 95.0 % after 10 days. A further supporting study according to OECD guideline 310 is available. The test showed a degradation of 84 % at the end of the test.
Saltwater
Supporting
The ready biodegradability of the test substance was tested in an OECD guideline study. This method employs no separate bacterial innoculum, and relies upon populations of bacteria which occur naturally in seawater. The overall assessment of biodegradability is based upon a comparison between experimentally determined oxygen consumption (BOD measurements) and the oxygen consumption predicted if all carbon present in the test material were completely oxidised (theoretical oxygen demand, ThOD). To enable an assessment of potential inhibitory effects of the test material (or its primary degradation products), an inhibition control is used, in which a mixture of the soluble reference compound and the test material is tested. The test substance biodegraded by 88% over 28 days and showed an inhibition of 15% to seawater bacteria. The oxygen blank was within formal limits of acceptability. The soluble reference material, sodium benzoate, degraded by more than 60% in the first ten days, indicating that the seawater used in the test contained a satisfactory population of viable bacteria.The test substance is ready biodegradable.
Conclusion: Four OECD guideline studies showed biodegradation rates of 84 % - 100 %. The substance is classified as readily biodegradable.
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