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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable study report which meets basic scientific principles

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1973
Report date:
1973

Materials and methods

Principles of method if other than guideline:
BASF test: Four groups of 5 mice per dose were treated intraperitoneally with preparations of the test substance in suitable vehicle. Group-wise documentation of clinical signs was performed over the 7-day study period. Body weight was determined before the start of the study only, as it was needed for determination of dose. The clinical signs and findings were reported in summary form. On the basis of the observed lethality, the LD50 value was estimated or determined using a graphical evaluation of the dose response curve on probability paper.
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Reference substance name:
Dicarboxylic Acid Mixture
IUPAC Name:
Dicarboxylic Acid Mixture
Details on test material:
- Name of test material (as cited in study report): Dicarboxylic Acid Mixture dest. (Bascal S)
- Composition of test material, percentage of components: ca. 30 % Succinic Acid, ca. 50 % Glutaric Acid, ca. 20 % Adipic Acid

Test animals

Species:
mouse
Strain:
not specified
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation:
500 mg/kg bw: males 18-27 g, females 19-24 g
400 mg/kg bw: males 22-29 g, females 20-25 g
320 mg/kg bw: males 26-30 g, females 21-23 g
250 mg/kg bw: males 25-29 g, females 21-25 g
200 mg/kg bw: males 25-29 g, females 22-24 g
160 mg/kg bw: males 26-29 g, females 21-23 g
125 mg/kg bw: males 25-29 g, females 21-26 g
100 mg/kg bw: males 25-30 g, females 22-25 g

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
CMC (carboxymethyl cellulose)
Details on exposure:
VEHICLE
- Concentration in vehicle (%): 2

DOSAGE PREPARATION
- Dose volume applied: 25, 20, 16, 12.5, 10, 8, 6.25 and 5 ml/kg bw for 500, 400, 320, 250, 200, 160, 125 and 100 mg/kg bw dose groups, respectively.
Doses:
100, 125, 160, 200, 250, 320, 400 and 500 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
120 mg/kg bw
Mortality:
see free text
Clinical signs:
dyspnea, staggering, reluctance to move, anorexia

Any other information on results incl. tables

Dose (mg/kg bw)

Sex

Mortalities in

1 h

24 h

48 h

7 days

500

M

0/5

3/5

3/5

5/5

F

0/5

5/5

5/5

5/5

400

M

0/5

2/5

3/5

5/5

F

0/5

2/5

/5

5/5

320

M

0/5

1/5

3/5

3/5

F

0/5

0/5

2/5

4/5

250

M

0/5

1/5

3/5

4/5

F

0/5

0/5

0/5

1/5

200

M

0/5

0/5

2/5

4/5

F

0/5

1/5

1/5

2/5

160

M

0/5

0/5

2/5

4/5

F

0/5

1/5

1/5

2/5

125

M

0/5

1/5

2/5

3/5

F

0/5

2/5

3/5

3/5

100

M

0/5

0/5

0/5

0/5

F

0/5

0/5

0/5

0/5

Applicant's summary and conclusion