2-dimethylaminoethyl methacrylate

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Original report is not available. However, the report is cited by a peer-reviewed OECD SIDS. Hence, the reliability was taken from the OECD report.

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Scenedesmus capricornutum

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Hardness:

30.3 mg/L

Test temperature:

22.8 - 23.2 °C

pH:

9.03 (start of the test); 7.90 (end of the test)

Dissolved oxygen:

9.13 - 9.25 mg/L

Nominal and measured concentrations:

Test concentrations (nominal): 10, 18, 32, 56 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 100 mL medium in a 300 mL conical flask.
- Initial cells density: 10 000 cell/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


GROWTH MEDIUM
- Standard medium used: yes (OECD Medium)


OTHER TEST CONDITIONS
- Light intensity and quality: 4000 - 5000 lux
- Photoperiod: 24h light per day

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

41.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 95% C.I. = 37.3 - 46.5 mg/L

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

69.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95% C.I. = 57.1 - 85.1 mg/L

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Measured concentrations of MADAME:

Nominal conc. (mg/L)	Measured conc. (mg/L)
	24 h	% of nominal	72 h	% of nominal
Control	N.D.	-	N.D.	-
10	8.82	88.2	N.D.	-
18	15.2	84.4	0.07	0.39
32	25.9	80.9	0.30	0.94
56	48.2	86.3	0.86	1.54
100	83.4	83.4	2.18	2.18

Nominal cell density:

Nominal conc. (mg/L)	Cell density (x 10000 cells/mL)
	0 h	24 h	48 h	72 h
Control	1.0	3.6	18.7	81.6
10	1.0	3.5	15.2	83.9
18	1.0	4.0	16.3	80.3
32	1.0	2.8	12.8	53.7
56	1.0	2.3	6.6	30.2
100	1.0	1.0	0.361	2.9

Validity criteria fulfilled:

yes

Description of key information

2-dimethylaminoethyl methacrylate is harmful to aquatic algae.

Key value for chemical safety assessment

EC50 for freshwater algae:

41.6 mg/L

Additional information

The determination of inhibitory effects of 2-dimethylaminoethyl methacrylate on Scenedesmus subspicatus was performed in a OECD Guideline 201 study (Alga, Growth Inhibition Test). The results of this study showed a EbC50 of 41.6 mg/L. The measurement of the growth inhibition showed a ErC50 value of 69.7 mg/L. Therefore, with high probability 2-dimethylaminoethyl methacrylate is harmful to aquatic algae.