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EC number: 219-784-2 | CAS number: 2530-83-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1987-09-09 to 1987-10-26
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- The restrictions were that only three strains of bacteria were used.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- only three strains used
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- [3-(2,3-epoxypropoxy)propyl]trimethoxysilane
- EC Number:
- 219-784-2
- EC Name:
- [3-(2,3-epoxypropoxy)propyl]trimethoxysilane
- Cas Number:
- 2530-83-8
- Molecular formula:
- C9H20O5Si
- IUPAC Name:
- trimethoxy[3-(oxiran-2-ylmethoxy)propyl]silane
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA97, TA98, TA100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor induced rat liver S9
- Test concentrations with justification for top dose:
- 8, 40, 200, 1000, 5000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Ethylene glycol dimethylether
- Justification for choice of solvent/vehicle: none given in report
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- TA 97 (without activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- TA 98 (without activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Sodium Azide
- Remarks:
- TA 100 (without activation)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- All strains (with activation)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Expression time (cells in growth medium): 48 hours
NUMBER OF REPLICATIONS: 3 plates per test concentration
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth; background lawn assessment - Evaluation criteria:
- Colony counts for test substance compared with solvent controls. A positive response is indicated by a two-fold increase in mean revertant numbers compared with the solvent control.
- Statistics:
- Mean values calculated from individual plate counts. Statistical significance calculated using F-test.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium, other: TA97
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- A statistically significant increase in revertants noted in Salmonella typhimurium strains TA 97 and TA 100 both in the absence and presence of S-9.
Any other information on results incl. tables
Table 2 : Mutagenicity assay, Number of revertants per plate (mean of 3 plates)
|
TA 97 |
TA 98 |
TA 100 |
||||||
Conc. |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
— MA |
+ MA |
Cytotoxic |
0* |
77.8 |
142.2 |
No |
18.2 |
43.4 |
No |
87.4 |
133.6 |
No |
8 |
62 |
123.7 |
No |
14 |
46.3 |
No |
96 |
141 |
No |
40 |
63.3 |
132 |
No |
18 |
34.5 |
No |
98.7 |
144.7 |
No |
200 |
57.3 |
135.7 |
No |
22 |
35.3 |
No |
120 |
169.7 |
No |
1000 |
70.7 |
168.3 |
No |
19.3 |
34.3 |
No |
243 |
301.3 |
No |
5000 |
154.7 |
276.7 |
No |
18.7 |
44.3 |
No |
598.7 |
690.7 |
No |
Positive Control |
340.8 |
357.8 |
No |
905 |
969.6 |
No |
502.8 |
432.2 |
No |
*solvent control with Ethylene glycol dimethylether
Applicant's summary and conclusion
- Conclusions:
- 3-Glycidoxypropyltrimethoxysilane (CAS 2530-83-8) (GLYMO) has been tested in a valid study according to a protocol that is similar to OECD TG 471 and under GLP. GLYMO produced a fivefold increase in revertants in Salmonella typhimurium TA 100, and a two fold increase in TA 97 with and without metabolic activation. The test substance is considered mutagenic in Salmonella typhimurium strains TA97 and TA100 both in the absence and presence of S-9 under the conditions of the test.
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