Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1964
Report date:
1964

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
other: BASF-test following internal SOP
GLP compliance:
no
Remarks:
pre-GLP
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Piperazine
EC Number:
203-808-3
EC Name:
Piperazine
Cas Number:
110-85-0
Molecular formula:
C4H10N2
IUPAC Name:
piperazine
Specific details on test material used for the study:
- Name of test material (as cited in study report): Piperazine, technical grade
- no further data

Test animals

Species:
mouse
Strain:
not specified
Sex:
male/female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
water
Doses:
25, 50, 100, 125, 160, 200, 1600 mg/kg bw
No. of animals per sex per dose:
25, 50, 100, 1600 mg/kg bw: 5 animals
125, 160, 200 mg/kg bw: 10 animals
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: several times on the day of application, at least once per day thereafter
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 125 mg/kg bw
Mortality:
1600 mg/kg dosing group: 5/5
200 mg/kg dosing group: 10/10
160 mg/kg dosing group: 9/10
late deaths reported
125 mg/kg dosing group: 6/10
100 mg/kg dosing group: 0/5
50 mg/kg dosing group: 0/5
25 mg/kg dosing group: 0/5
Clinical signs:
following substance administration: slightly unsteady gait, intermitted respiration, shrunken flanks
following days: apathy, scrubby coat, crusted eyes, survivors recovered till day 12, the animals of the lower dosing group (25-100 mg/kg) appeared normal on day 1 after application.
Body weight:
no data
Gross pathology:
deceased animals: strong gastrointestinal irritation, pale livers, hyperaemia in the accessory sex glands

Applicant's summary and conclusion

Conclusions:
LD50 for intraperitoneal route is 125 mg/kg bw.
Executive summary:

Rats were dosed with piperazine by intraperitoneal injection at 7 doselevels. The LD50 was 125 mg/kg bw.

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