Registration Dossier
Registration Dossier
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EC number: 203-808-3 | CAS number: 110-85-0
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 964
- Report date:
- 1964
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- other: BASF-test following internal SOP
- GLP compliance:
- no
- Remarks:
- pre-GLP
- Limit test:
- no
Test material
- Reference substance name:
- Piperazine
- EC Number:
- 203-808-3
- EC Name:
- Piperazine
- Cas Number:
- 110-85-0
- Molecular formula:
- C4H10N2
- IUPAC Name:
- piperazine
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Piperazine, technical grade
- no further data
Test animals
- Species:
- mouse
- Strain:
- not specified
- Sex:
- male/female
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- water
- Doses:
- 25, 50, 100, 125, 160, 200, 1600 mg/kg bw
- No. of animals per sex per dose:
- 25, 50, 100, 1600 mg/kg bw: 5 animals
125, 160, 200 mg/kg bw: 10 animals - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: several times on the day of application, at least once per day thereafter
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 125 mg/kg bw
- Mortality:
- 1600 mg/kg dosing group: 5/5
200 mg/kg dosing group: 10/10
160 mg/kg dosing group: 9/10
late deaths reported
125 mg/kg dosing group: 6/10
100 mg/kg dosing group: 0/5
50 mg/kg dosing group: 0/5
25 mg/kg dosing group: 0/5 - Clinical signs:
- following substance administration: slightly unsteady gait, intermitted respiration, shrunken flanks
following days: apathy, scrubby coat, crusted eyes, survivors recovered till day 12, the animals of the lower dosing group (25-100 mg/kg) appeared normal on day 1 after application. - Body weight:
- no data
- Gross pathology:
- deceased animals: strong gastrointestinal irritation, pale livers, hyperaemia in the accessory sex glands
Applicant's summary and conclusion
- Conclusions:
- LD50 for intraperitoneal route is 125 mg/kg bw.
- Executive summary:
Rats were dosed with piperazine by intraperitoneal injection at 7 doselevels. The LD50 was 125 mg/kg bw.
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