Piperazine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1964

Report date:

1964

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

BASF-test following internal SOP according to Smyth, H.F. et al.: Am. Ind. Hyg. Ass. J. 23, 95-107:
This test (also called inhalation hazard test) was performed in principle as described in an earlier version of the OECD Guideline 403. It demonstrates the toxicity of an atmosphere saturated with vapours of the volatile components of a test substance at the temperature chosen for vapour generation (20 °C).

GLP compliance:

no

Remarks:

pre-GLP

Test type:

other: inhalation hazard test

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Piperazine

EC Number:

203-808-3

EC Name:

Piperazine

Cas Number:

110-85-0

Molecular formula:

C4H10N2

IUPAC Name:

piperazine

Specific details on test material used for the study:

- Name of test material (as cited in study report): Piperazine, technical grade
- no further data

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Mean weight at study initiation: 179.6 g
- Fasting period before study: none
- Diet ad libitum, feed was withdrawn during the time of exposure
- Water ad libitum
- Acclimation period: no data

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

Several groups of usually 3 rats per sex were exposed sequentially to the vapours, generated by bubbling 200 l/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 8 hours. The exposure time not causing lethality was usually tested twice.
No analytical determination of the atmosphere concentrations was performed. The nominal concentration usually can be calculated as quotient of the amount of test substance weight loss during the exposure, which is given in the raw data, and the amount of air used during the exposure.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

8 h

Concentrations:

1.61 mg/l

No. of animals per sex per dose:

3 males and 3 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations: at least once daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC0

Effect level:

1.61 mg/L air (nominal)

Exp. duration:

8 h

Remarks on result:

other: IRT

Effect levels 2

Sex:

male/female

Dose descriptor:

LC0

Effect level:

2 mg/L air (nominal)

Exp. duration:

4 h

Remarks on result:

other: Extrapolated using Habers law

Mortality:

no deaths occurred

Clinical signs:

other: slight mucosal irritation was reported

Body weight:

nothing abnormal reported

Gross pathology:

organs without findings

Any other information on results incl. tables

No mortality was observed when 6 rats were exposed for 8 hours to an atmosphere that has been saturated at room temperature with the volatile part of the compound.

Applicant's summary and conclusion

Conclusions:

LC0 for 4h exposure is 2 mg/l.