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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
04 Feb 1974 - 12 Feb 1974
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report Date:
1975

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Principles of method if other than guideline:
The test was performed according to the principles described in OECD Guideline 403.
The test demonstrates the toxicity of an atmosphere saturated with vapors of the volatile components of a test substance at the temperature chosen. The documentation of clinical signs was performed over a period of 7 days. In order to verify the results, the test was repeated once.
GLP compliance:
no
Test type:
other: Inhalation hazard test
Limit test:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Methylentriglykol. Test substance No. 88/289
- Physical state: liquid
- Analytical purity: ca. 95%

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: male: 172g (mean), female: 161g (mean)

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
not specified
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Temperature in air chamber and for vapour generation: 20°C
- 3 rats per sex were exposed sequentially to the vapour.
- vapour generated by bubbling 200l/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder.
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
8 h
Concentrations:
0.15 mg/l
No. of animals per sex per dose:
6
Control animals:
yes
Details on study design:
- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC0
Effect level:
> 10 ppm
Exp. duration:
8 h
Remarks on result:
other: figure nominally represents the saturated vapour concentration
Mortality:
No mortality was observed at saturated vapour concentration.
Clinical signs:
other: No clinical signs were observed.
Body weight:
male: 200g (mean)
female: 180g (mean)
Gross pathology:
No abnomalities were found at necropsy.

Any other information on results incl. tables

No clinical signs of toxicity or mortality were observed after an 8h inhalation of an atmosphere saturated with vapours of the volatile components of the test substance.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Executive summary:

In a vapour inhalation acute toxicity test carried out following the principles of the OECD guideline, male and female rats were exposed to a saturated vapour concentration of the test substance 2 -(2 -(2 -methoxyethoxy)ethoxy)ethanol for a period of 8 hours. No clinical signs of toxicity were reported. It can be concluded that the test substance does not present an acute inhalation hazard.