Registration Dossier
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EC number: 203-962-1 | CAS number: 112-35-6
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Substance: 2-(2-(2-methoxyethoxy)ethoxy)ethanol:
Leuciscus idus: 96hr LC0>10000mg/l
Danio rerio: 96hr LC0>5000mg/l
P promelas, 96hr LC50>10000mg/l
Danio rerio, 96hr NOEC (fish embryo test)>820mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 10 000 mg/L
Additional information
Conventional guideline acute toxicity studies are available for 2 -(2 -(2 -methoxyethoxy)ethoxy)ethanol across two different fish species. Studies consistently show that the substance is effectively non toxic to fish with LC0 values between 5000 -10000mg/l and LC50 values in excess of 10000mg/l. There is no data available on marine fish.
2-(2 -(2 -methoxyethoxy)ethoxy) ethanol (TEGME) and its main metabolite (2 -(2 -methoxyethoxy)ethoxy) acetic acid (TEGMEA) were evaluated using the zebrafish embryotoxicity test (ZET). The morphological characteristics of each embryo were assessed at 72 and 96 hours post fertilization (hpf) following exposure to the test substance at various concentrations up to a maximum tolerated dose ascertained by a dose range finder study. At 72 hpf, the embryos were evaluated for dead or alive. At 96 hpf, the embryos and larvae were evaluated for a wide series of normal or anomalous developmental characteristics substance at various concentrations up to a maximum tolerated dose ascertained by a dose range finder study, which in the case of TEGME was 10mM and TEGMEAA 5mM. The lower figure was used for the main study for both substances.
Both substances were found to cause some isolated effects across all doses in both the range finder and main study. There was no dose response or consistency in the findings with the highest doses showing the least effects. The findings were interpreted as random and not treatment related. The positive controls (ethanol and methoxyacetic acid) used exhibited a wider range of developmental effects (growth retardation and malformations). Both substances were considered as negative by the study authors in this assay. The NOAEL was 5.0mM (820mg/L) for TEGME and 5mM (890mg/L) for TEGMEA, the maximum tested doses.
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