2-ethoxyethanol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: acceptable publication

Data source

Reference

Reference Type:

publication

Title:

The results of microbial mutation test for forty-three industrial chemicals

Author:

Shimizu et al..

Year:

1985

Bibliographic source:

Jpn. J. Ind. Health 27, 400-419

Materials and methods

Principles of method if other than guideline:

Method: other: similar to OECD Guideline 471

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-ethoxyethanol

EC Number:

203-804-1

EC Name:

2-ethoxyethanol

Cas Number:

110-80-5

Molecular formula:

C4H10O2

IUPAC Name:

2-ethoxyethan-1-ol

Details on test material:

purity in all test = 98 %
Name of test substance as stated in publication: 2-Ethoxyethanol

Method

Species / strain

Species / strain / cell type:

other: Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538

Metabolic activation:

with and without

Metabolic activation system:

S9 from liver of male Sprague-Dawley rats

Test concentrations with justification for top dose:

5, 10, 50, 10, 500, 1000, 5000 µg/plate

Controls

Controls 1

Positive controls:

yes

Positive control substance:

benzo(a)pyrene

Controls 2

Positive controls:

yes

Positive control substance:

9-aminoacridine

Controls 3

Positive controls:

yes

Positive control substance:

N-ethyl-N-nitro-N-nitrosoguanidine

Controls 4

Positive controls:

yes

Positive control substance:

4-nitroquinoline-N-oxide

Controls 5

Positive controls:

yes

Positive control substance:

2-nitrofluorene

Controls 6

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene

Controls 7

Positive controls:

yes

Positive control substance:

furylfuramide

Details on test system and experimental conditions:

OTHER: Ames Test

Results and discussion

Test results

Metabolic activation:

with and without

Genotoxicity:

negative

Remarks on result:

other: other: Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538; Escherichia coli WP2uvrA

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

According to the results in this gene mutation assay the test substance 2-ethoxyethanol was not mutagenic to the microbial strains of Salmonella typhimurium (TA 98, TA 100, TA 1535, TA 1537, TA 1538) and Escherichia coli (WP2uvrA).