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EC number: 203-804-1 | CAS number: 110-80-5
Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Effect of dose on the disposition of 2-ethoxyethanol after inhalation by F344/N rats
- Author:
- Kennedy CH, Bechtold WE, Chang I-Y, Henderson RF.
- Year:
- 1 993
- Bibliographic source:
- Fundamen. Appl. Toxicol. 21(4), 486-491
Materials and methods
- Type of study / information:
- Type: inhalative absorption
- Principles of method if other than guideline:
- Method: other: metabolism analysis in rats
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2-ethoxyethanol
- EC Number:
- 203-804-1
- EC Name:
- 2-ethoxyethanol
- Cas Number:
- 110-80-5
- Molecular formula:
- C4H10O2
- IUPAC Name:
- 2-ethoxyethan-1-ol
- Details on test material:
- Name of the test substance as stated in the publication: 2-ethoxyethanol
radiolabeled 2-ethoxyethanol: purchased from Amersham Laboratories (Buckinghamshire, UK);
unlabeled 2-ethoxyethanol: purchased from Aldrich Chemical Co. (Milwaukee, WI), purity 99+ %;
Constituent 1
Results and discussion
Any other information on results incl. tables
The actual exposure concentrations were 5.03 +/-0.23 ppm and 46.0 +/-2.2 ppm.
Absorption and retention of [14C]-2 -ethoxyethanol:
| Exposure 5.0 ppm | Exposure 46.0 ppm | |
| Volume inhaled (L) | 132 +/-3 | 138 +/-6 |
| 2 -ethoxyethanol inhaled (µmol/ppm) | 4.41 +/-0.10 | 4.61 +/-0.20 |
| Body burdon (total body burdon at end of exposure, µmol/ppm) | 1.23 +/-0.08 | 1.46 +/-0.02 |
| Total excreta (µmol/ppm) | 1.31 +/-0.06 | 1.39 +/-0.04 |
| Amount retained (µmol/ppm) | 1.25 +/-0.04 | 1.33 +/-0.04 |
| Percentage retained | 28 +/-1.1 | 29 +/-1.6 |
Excretion of [14C]-2 -ethoxyethanol equivalents:
| µmol [14C]-2 -ethoxyethanol equivalents | ||||
| Route of excretion | 5 ppm | % | 46 ppm | % |
| 14CO2 during exposure | 1.4 | 21 | 14 | 22 |
| 14CO2 postexposure | 1.1 +/-0.1 | 16 | 10 +/-1 | 16 |
| Urine | 3.1 +/-0.1 | 46 | 29 +/-1 | 46 |
| Feces | 0.04 +/-0.01 | 0.6 | 1.0 +/-0.2 | 1.6 |
| Exhaled 2 -ethoxyethanol postexposure | 0.23 +/-0.09 | 4.2 | 2.4 +/-0.3 | 3.7 |
| Carcass | 0.74 +/-0.06 | 11 | 6.6 +/-0.2 | 10 |
| Total | 6.6 +/-0.3 | 100 | 64 +/-2 | 100 |
4 urinary metabolites were detected from which three were identified by means of reverse-phase HPLC. The identified metabolites were ethylene glycol, ethoxyacetic acid and the glycine conjugate of ethoxyacetic acid (comparison via HPLC of authentic standards and GC/MS analysis).
Applicant's summary and conclusion
- Conclusions:
- The major urinary metabolites identified in male F344/N rats inhalatively exposed to 5 or 46 ppm of radiolabeled 2-ethoxyethanol were ethylene glycol, ethoxyacetic acid and the glycine conjugate of ethoxyacetic acid. An other major excretion route of radiolabeled 2-ethoxyethanol equivalents was the exhalation of 14CO2.
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