Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Additional toxicological data

Currently viewing:

Administrative data

additional toxicological information
Type of information:
experimental study
Adequacy of study:
other information
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable publication

Data source

Reference Type:
Effect of dose on the disposition of 2-ethoxyethanol after inhalation by F344/N rats
Kennedy CH, Bechtold WE, Chang I-Y, Henderson RF.
Bibliographic source:
Fundamen. Appl. Toxicol. 21(4), 486-491

Materials and methods

Type of study / information:
Type: inhalative absorption
Principles of method if other than guideline:
Method: other: metabolism analysis in rats
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
Name of the test substance as stated in the publication: 2-ethoxyethanol
radiolabeled 2-ethoxyethanol: purchased from Amersham Laboratories (Buckinghamshire, UK);
unlabeled 2-ethoxyethanol: purchased from Aldrich Chemical Co. (Milwaukee, WI), purity 99+ %;

Results and discussion

Any other information on results incl. tables

The actual exposure concentrations were 5.03 +/-0.23 ppm and 46.0 +/-2.2 ppm.

Absorption and retention of [14C]-2 -ethoxyethanol:

   Exposure 5.0 ppm  Exposure 46.0 ppm
 Volume inhaled (L)  132 +/-3  138 +/-6
 2 -ethoxyethanol inhaled (µmol/ppm)  4.41 +/-0.10  4.61 +/-0.20
 Body burdon (total body burdon at end of exposure, µmol/ppm)  1.23 +/-0.08  1.46 +/-0.02
 Total excreta (µmol/ppm)  1.31 +/-0.06  1.39 +/-0.04
 Amount retained (µmol/ppm)  1.25 +/-0.04  1.33 +/-0.04
 Percentage retained  28 +/-1.1  29 +/-1.6

Excretion of [14C]-2 -ethoxyethanol equivalents:

            µmol [14C]-2 -ethoxyethanol equivalents
Route of excretion   5 ppm  %  46 ppm  %
 14CO2 during exposure  1.4  21  14  22
 14CO2 postexposure  1.1 +/-0.1  16  10 +/-1  16
 Urine  3.1 +/-0.1  46  29 +/-1  46
 Feces  0.04 +/-0.01  0.6  1.0 +/-0.2  1.6
 Exhaled 2 -ethoxyethanol postexposure  0.23 +/-0.09  4.2  2.4 +/-0.3  3.7
 Carcass  0.74 +/-0.06  11  6.6 +/-0.2  10
 Total  6.6 +/-0.3  100  64 +/-2  100

4 urinary metabolites were detected from which three were identified by means of reverse-phase HPLC. The identified metabolites were ethylene glycol, ethoxyacetic acid and the glycine conjugate of ethoxyacetic acid (comparison via HPLC of authentic standards and GC/MS analysis).

Applicant's summary and conclusion

The major urinary metabolites identified in male F344/N rats inhalatively exposed to 5 or 46 ppm of radiolabeled 2-ethoxyethanol were ethylene glycol, ethoxyacetic acid and the glycine conjugate of ethoxyacetic acid. An other major excretion route of radiolabeled 2-ethoxyethanol equivalents was the exhalation of 14CO2.