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EC number: 211-776-7 | CAS number: 694-83-7
Table 7.2.1 : Weight evolution of Guinea Pigs after both treatments
Time after treatment started
% gain or loss in weight
Wed ¿ 16-29-47 Subcu. Inj. started
11-11 to 11-19 inc. Rest Period
Thurs 11-20 Final Patch
The skin sensitisation potential of Hexamethylene Diamine (HMD) at 2% and 1% was tested (Hardy, 1947b). Experiment was realized with 10 Guinea Pig.
A 2% HMD aqueous solution was applicated (epicutaneous route) on back (near shoulder) of clipped Guinea Pig.Three successive applications were performed the first day on the same area . Then, six successive subcutaneous injections of solution of 1% HMD were performed on the left side through 13 days followed by a 8-day rest period. Then the challenge (epicutaneous and intradermal routes) was performed with 2% and 1% HMD aqueous solution. Skin reactions were analysed. Finally Guinea Pigs were sacrified with Illum gas two days after final injection (challenge) and a gross-autopsy was performed for some animals. The body weight gain was measured.
Animals showed necrotic area at site of injection of HMD 3 days after treatment. Swelling occured at site of injection at 6 days post treatment. Gross-autopsy was performed for three Guinea Pig.
After initial patch application, within ten minutes, G. Pigs began hopping around cage definite erythema on left side. One hour after application, HMD showed signs of becoming necrotic + dark brown. Three hours post application, application site was definitely necrotic dark brown. At the end of the experiment, necrotic areas beginning to slough off slightly.
Under the test conditions, necrosis was observed 24-hour after the last injections following the final patch application. Based on these results, no conclusion can be made.
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