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EC number: 211-776-7 | CAS number: 694-83-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- Published: 2010
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- unsuitable test system
Data source
Reference
- Reference Type:
- publication
- Title:
- Cytotoxicity, cellular uptake, glutathione and DNA interactions of an antitumor large-ring PtII chelate complex incorporating the cis-1,4-diaminocyclohexane carrier ligand
- Author:
- Kasparkova, J.; Suchankova, T.; Halamikova, A.; Zerzankova, L.; Vrana, O.; Margiotta, N.; Natile, G.; Brabec, V
- Year:
- 2 010
- Bibliographic source:
- Biochemical Pharmacology, 79, 552-564
Materials and methods
- Objective of study:
- other: cellular uptake
- Principles of method if other than guideline:
- basic research experiment: Cellular uptake of PtCl2(cis-1,4-diaminocyclohexane) into A2780 cells (i.e. Human ovarian carcinoma cells, sensitive to cisplatin) was measured. Therefore, cells were seeded (in 60 mm cell culture dishes with 30 000 cells per cubic centimetre). After over night incubation the cells were exposed to medium containing the platinum compound at a concentration of 2.7 microM for either 4 or 24 hours. After the treatment period cells were washed twice with ice-cold phosphate buffered saline, harvested via trypsin-detachment, pelleted and stored at minus 80 °C. For the assay pellets wer digested with 12 M HCl and platinum content was determined via flameless atomic absorption spectrophotometry (FAAS). Cellular uptake measures were corrected for adsorption effects and experiments were performed in quadrudplicate.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- PtCl2(cis-1,4-diaminocyclohexane)
- IUPAC Name:
- PtCl2(cis-1,4-diaminocyclohexane)
- Test material form:
- not specified
- Details on test material:
- - Name of test material (as cited in study report):
- Analytical purity: >95%
Constituent 1
- Radiolabelling:
- not specified
Results and discussion
Applicant's summary and conclusion
- Conclusions:
- PtCl2(cis-1,4-diaminocyclohexane) was taken up into human ovarian carcinoma cells (A2780). After 4 and 24 hours 4.7 and 38 pmol compund/10EXP6 cells was found respectively, i.e. 1.3 to 2fold the amount of cisplatin which was taken up by these cells within the same timeframe.
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