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EC number: 211-776-7 | CAS number: 694-83-7
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- For details on endpoint specific justification please see read-across report in section 13 or find a link in cross-reference “assessment report”.
- Reason / purpose for cross-reference:
- assessment report
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 43.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CI: 9.4-91.7
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 182 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CI: 84.7-282
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 558 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CI: 966-3760
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 26.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CI: 1.8-72.4
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 341 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CI: 137-1787
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 291 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Remarks on result:
- other: 95% CI: 98.7-978
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- 1 574 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Remarks on result:
- other: 95% CI: 583-76327
- Details on results:
- Due to the very similar molecular weight of source and target substance, the effect concentrations for the source substance are also valid for the target substance without further adjustment.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an activated sludge respiration inhibition test according to OECD 209 (2010) with the source substance 1,6-hexane diamine (HMD; CAS No. 124-09-4), nitrification proved to be the most sensitive endpoint (highest test item concentration: 1000 mg/L):
EC50 (3h; total respiration): 1558 mg/L;
EC50 (3h; heterotrophic respiration): >> 1000 mg/L;
EC50 (3h; nitrification): 291 mg/L.
Because of the very similar molecular weight of source and target substance, these results are directly applicable to the target substance DCH. - Executive summary:
The study used as source investigated the STP microorganism toxicity according to OECD TG 209 (2010). The study results of the source compound were considered applicable to the target compound. Justification and applicability of the read-across approach (structural analogue) is outlined in the read-across report in section 13 or find a link in cross reference “assessment report”.
Reference
Description of key information
In an activated sludge respiration inhibition test according to OECD 209 (2010) with the source substance 1,6-hexane diamine (HMD; CAS No. 124-09-4), nitrification proved to be the most sensitive endpoint (highest test item concentration: 1000 mg/L):
EC50 (3h; total respiration): 1558 mg/L;
EC50 (3h; heterotrophic respiration): >> 1000 mg/L;
EC50 (3h; nitrification): 291 mg/L.
Because of the very similar molecular weight of source and target substance, these results are directly applicable to the target substance DCH.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 291 mg/L
Additional information
The study used as source investigated the STP microorganism toxicity according to OECD TG 209 (2010). The study results of the source compound were considered applicable to the target compound. Justification and applicability of the read-across approach (structural analogue) is outlined in the read-across report in section 13.
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