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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP guideline study
Qualifier:
according to guideline
Guideline:
other: EEC directive 79/831 EEC, Annex V, C2
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the higest test concentration (nominal 500 mg/L) was used as a stock solution. This test medium was serially diluted with culture medium to obtain the desired test concentrations (31.25, 62.5, 125, 250, 500 mg/L). A control, containing culture medium only was tested in parallel.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 hours
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Test temperature:
19 - 21 °C
pH:
7.8 - 8.1
Dissolved oxygen:
7.0 - 9.2 mg/L
Nominal and measured concentrations:
Nominal test concentrations (mg/L): 31.25, 62.5, 125, 250, 500 and control
Details on test conditions:
TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Fill volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the test guideline DIN 38412, L 11

OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours, diffuse light (ca. 5 µE/m²*s)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24, 48 h
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
24 hour ECx values: EC0: 500 mg/L EC50: > 500 mg/L EC100: > 500 mg/L
Reported statistics and error estimates:
No statistical analysis of the data was performed. The EC0, the EC50 and the EC100 were determined directly from the raw data.
Validity criteria fulfilled:
yes
Conclusions:
The test revealed a no-effect concentration (EC0) of 500 mg/L, whereas an EC50 of >500 mg/L was determined.
Executive summary:

An acute toxicity test with Daphnia magna was performed according to EEC directive 79/831 EEC, Annex V, C2. Thr following nominal test concentrations 31.25, 62.5, 125, 250, 500 mg/L and a control were investigated. As effect parameter the mobility after 0, 3, 6, 24, 48 h was measured. The test revealed a no-effect concentration (EC0) of 500 mg/L, whereas an EC50 of >500 mg/L was determined.

Description of key information

The key value for chemical safety assessment was experimentally determined by BASF AG (1988). An acute toxicity test with Daphnia magna was performed according to EEC directive 79/831 EEC, Annex V, C2.
The test revealed a no-effect concentration (EC0) of 500 mg/L, whereas an EC50 of >500 mg/L was determined.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
500 mg/L

Additional information

The acute toxicity of 2 -Pyrrolidone towards aquatic invertebrates was determined according to Directive 79/831/EEC, Annex V, C2 (1984) with Daphnia magna as test organism (BASF AG, 1988). In this static freshwater test, nominal substance concentrations of 0 (control), 31.25, 62.5, 125, 250, and 500 mg/L were tested. In the test vessels, 5 animals were exposed to 10 mL test medium for 48 hours, respectively. 4 replicates per test concentration were used. As final results, the EC0 (48 h) amounts to 500 mg/L while the EC50 (48 h) was determined to be > 500mg/L.