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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

Currently viewing:

Administrative data

Endpoint:
in vitro gene mutation study in mammalian cells
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: no informtion on GLP. Study report lacks some details but nevertheless provides useful supporting information

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
GLP compliance:
not specified
Type of assay:
mammalian cell gene mutation assay

Test material

Constituent 1
Reference substance name:
68334-30-5
Cas Number:
68334-30-5
IUPAC Name:
68334-30-5
Constituent 2
Reference substance name:
Diesel Fuel
IUPAC Name:
Diesel Fuel
Test material form:
other: low viscosity liquid hydrocarbon
Details on test material:
Diesel fuel

Method

Target gene:
L5178Y mouse lymphoma cells
Test concentrations with justification for top dose:
0.125, 0.188, 0.25, 0.375, 0.5 uL/ml - non activation study
0.064, 0.125, 0.188, 0.25, 0.375, 0.5 uL/ml - activation study
Vehicle / solvent:
acetone
Controls
Untreated negative controls:
no
Negative solvent / vehicle controls:
yes
Remarks:
ACETONE
Positive controls:
yes
Positive control substance:
other: ethylmethane sulphonate and dimethyl nitrosamine
Details on test system and experimental conditions:
L5178Y MOUSE LYMPHOMA CELLS

Results and discussion

Test results
Species / strain:
mouse lymphoma L5178Y cells
Metabolic activation:
with and without
Genotoxicity:
negative
Remarks:
both with and without activation
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

Diesel fuel was not mutagenic in the mouse lymphoma assay
Executive summary:

The genotoxic activity of diesel fuel was evaluated in a mouse lymphoma assay using L5178Y cells. Assays both in the presence and absence of a mouse liver mirosome fraction were conducted. Dose levels ranged between 0.064 and 0.5 uL/ML.

Results showed no evidence of of genotoxic activity, either in the presence or absence of metabolic activation. The positive control materials both showed expected results.