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Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Endpoint:
adult fish: sub(lethal) effects
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1979
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented with basic data given.

Data source

Reference
Reference Type:
publication
Title:
Structure Related Update of Chlorinated Paraffins in Bleak (Alburnus alburnus L).
Author:
Bengtsson, B.E., Svanberg, O. and Linden, E.
Year:
1979
Bibliographic source:
AMBIO Vol. 8, No. 2-3, pp. 121-122.

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Paraffin waxes and Hydrocarbon waxes, chloro
EC Number:
264-150-0
EC Name:
Paraffin waxes and Hydrocarbon waxes, chloro
Cas Number:
63449-39-8
Molecular formula:
C18H33Cl5 C18H30Cl8 C20H36Cl6 C20H33Cl9 C25H45Cl7 C25H42Cl10 C25H29C23 C30H53Cl9 C30H49Cl13 C30H35Cl27
IUPAC Name:
Paraffin waxes and Hydrocarbon waxes, chloro
Details on test material:
C18-26, 49% Cl (liquid). Witachlor 549

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
No data

Test solutions

Vehicle:
not specified
Details on test solutions:
Prepared from the commercially available test substance dissolved in acetone and added to the water. The initial concentration of acetone in the aquarium was 0.1 ml/L water.

Test organisms

Test organisms (species):
Alburnus alburnus
Details on test organisms:
TEST ORGANISM
- Source: Bleaks (Alburnus alburnus L) caught by dip net in the Tvaren Bay (about 80 km south of Stockholm in the Baltic Sea)
- Weight at study initiation: 4.5 g
- Acclimation: three to five months

FEEDING DURING TEST
- Frequency: Not fed during experiment

Study design

Test type:
semi-static
Water media type:
saltwater
Total exposure duration:
14 d
Post exposure observation period:
No data

Test conditions

Hardness:
No data
Test temperature:
10 degrees C
pH:
No data
Dissolved oxygen:
No data
Salinity:
7‰
Details on test conditions:
TEST SYSTEM
- Test vessel: 70L glass aquarium, containing 60L of natural Baltic Sea water (salinity 7‰).
- Material, size, headspace, fill volume: The test solution, containing 125 ug test substance/L water
- Renewal rate of test solution (frequency/flow rate): every second or third day (at weekends)

- For chemical analysis, pooled samples of five fishes, which were sacrificed and rinsed in 100mL of distilled water per fish during 30 seconds, were wrapped in aluminum foil and kept frozen at -20 degrees C until analysis. 15 bleaks were exposed during 14 days to 125 ug/L of the test substance in the water. At the end of the exposure, five fishes were sampled and placed in uncontaminated Baltic sea water. This water was renewed with the same frequency as the test solution. Additional samples of fish were taken after one and 7 days in the contaminated water.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
> 0.125 mg/L

Any other information on results incl. tables

No mortality or behavioral effects.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No mortality or effect on behaviour was seen in fish exposed to the LCCP during the test. NOEC > 0.125 mg/L.
Executive summary:

No mortality or effect on behaviour was seen in fish exposed to the LCCP during the test. NOEC > 0.125 mg/L.