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Environmental fate & pathways

Biodegradation in water: screening tests

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biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)

Data source

Referenceopen allclose all

Reference Type:
Environmental Risk Evaluation Report: Long-Chain Chlorinated Paraffins
UK Environment Agency
Reference Type:
Uptake of chlorinated paraffins and PCB [Polychlorinated Biphenyl] from suspended solids and food by juvenile Atlantic salmon.
Zitko, V.
Bibliographic source:
Bulletin of Environmental Contamination and Toxicology, 12, 406-412. In: UK Environment Agency. Environmental Risk Evaluation Report: Long-Chain Chlorinated Paraffins. January 2009.
Reference Type:
Fate of high molecular weight-chlorinated paraffins in the aquatic environment.
Zitko V and Arsenault E
Bibliographic source:
Advances in Environmental Science and Technology, 8, 409-418.

Materials and methods

Principles of method if other than guideline:
Amounts of LCCP in both stoppered and aerated flasks in spiked sediment/water solutions were measured over a period of 28 days.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Paraffin waxes and Hydrocarbon waxes, chloro
EC Number:
EC Name:
Paraffin waxes and Hydrocarbon waxes, chloro
Cas Number:
Molecular formula:
C18H33Cl5 C18H30Cl8 C20H36Cl6 C20H33Cl9 C25H45Cl7 C25H42Cl10 C25H29C23 C30H53Cl9 C30H49Cl13 C30H35Cl27
Paraffin waxes and Hydrocarbon waxes, chloro
Details on test material:
C20-30, 42% wt. Cl

Study design

Oxygen conditions:
other: aerobic and anaerobic
Inoculum or test system:
natural water / sediment
Details on inoculum:
The tests were carried out in flasks containing 25 g of sediment, 300 ml of sea water and 10 ml of a suspension of decomposing organic
matter in sea water. Three flasks were used in the experiment, one for each of the two products tested, and a third acting as control. The flasks were incubated at 19–22°C with aeration for 28 days (the volume of water was kept constant by adding distilled water during the test). In addition to these, another identical set of three flasks were kept stoppered over the exposure period.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
596 other: mg/kg dry weight sediment
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
test mat. analysis

Results and discussion

Details on results:
Concentration of chlorinated paraffin (mg/kg dry weight)
Aerated Flasks
0 days: 596
10 days: 257
21 days: 147
28 days: 377

Stoppered Flasks:
0 days: 596
10 days: 80
21 days: 194
28 days: 98

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Interpretation of results:
inherently biodegradable, not fulfilling specific criteria
Test material was consumed in both the aerated and stoppered flasks over 28 days.
Executive summary:

Test material was consumed in both the aerated and stoppered flasks over 28 days.

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