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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Guideline study, however DMSO was used as solubliser

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: OECD guideline 202
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMSO

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Feeding during test: daily
- Food type: green algae

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d

Test conditions

Test temperature:
20 - 22 ± °1C
Nominal and measured concentrations:
Nominal test concentrations: 3-300 mg/l, control of DMSO:HCO-40= 9:1 (100 mg/kg) and laboratory water control.
Details on test conditions:
TEST SYSTEM
- Test vessel: beaker (500 ml)
- Fill volume: 400 ml
- Renewal rate of test solution (frequency/flow rate): renewal of test substance every 48 hours.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate:

OTHER TEST CONDITIONS
- Photoperiod: 8:16 h day night regime

VEHICLE CONTROL PERFORMED: yes

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
4.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: The test is classified as disregarded study since DMSO was used as solubiliser. DMSO is known to increase the toxicity of substances.
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
9.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: The test is classified as disregarded study since DMSO was used as solubiliser. DMSO is known to increase the toxicity of substances.
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mobility and reproduction
Remarks on result:
other: The test is classified as disregarded study since DMSO was used as solubiliser. DMSO is known to increase the toxicity of substances.
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
9.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality and reproduction
Remarks on result:
other: The test is classified as disregarded study since DMSO was used as solubiliser. DMSO is known to increase the toxicity of substances.

Applicant's summary and conclusion