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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Description of key information

- Freshwater:
EC 50 = 0.73 mg/L (OECD 202, Daphnia magna, 48 h)
- Marine water
EC50 = 0.27 mg/L (Mytilus edulis)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.73 mg/L

Marine water invertebrates

Marine water invertebrates
Effect concentration:
0.27 mg/L

Additional information

In an acute toxicity test on Daphnia magna performed according to OECD 202, an EC50 value of 0.73 mg/L based on PAA was determined using an equilibrium PAA concentration of 5.2 % (Gardner and Bucksath, 1996). The PAA concentration was monitored indirectly by measuring the hydrogen peroxide concentration which revealed an decrease in concentration ranging between 19 and 35 % during the test.


Five other static and one semi-static acute toxicity tests with diluted equilibrium PAA (4.5 - 15.5 %) in the fresh water flea Daphnia magna have been reported without chemical analysis. The endpoints of these tests were based on nominal peracetic acid concentrations. The 48-hour EC50 values of these tests with equilibrium products ranged between 0.48 and 1.94 mg /L based on PAA.


Two studies (Licata-Messana, 1995; Panouillères et al., 2007) have been disregarded for risk assessment because the amount of peracetic acid in the products applied was very low (0.35% and 0.15% PAA vs. 7% and 3% H2O2, respectively). It is likely that the toxicity of other components such as hydrogen peroxide, which was present at a 20 times higher concentration than PAA, were mainly responsible for the toxicity observed.


In addition to the freshwater studies mentioned above, several studies were done with salt water invertebrates. Salt water studies were conducted with brown shrimp (Crangon crangon), pacific oyster (Crassostrea gigas) and the common mussel (Mytilus edulis), (Tinsley and Sims, 1987; Butler, 1987 and Fairhurst, 1987). E(L)C50 values were 15, 0.28 and 0.27 mg/L based on PAA, respectively. Consequently, the EC50 of 0.27 mg/L for the mussel Mytilus edulis, is used as key values for marine invertebrates.