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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

3h EC50 (activated sludge, domestic) = 135 mg/L (respiration inhibition test, similar to OECD 209)

Key value for chemical safety assessment

EC50 for microorganisms:
135 mg/L

Additional information

The study with the read-across substance C12AS Na (CAS 151-21-3) using activated sludge from a municipal sewage treatment plant (Dutka 1983) was chosen as a key study. Since preference is given to tests with a mixed inoculum rather than tests based on single species, a read-across to another structurally related category members was conducted in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5. The non-GLP test with C12AS Na was performed according to a guideline similar to OECD 209. The EC50 values of 188 and 135 mg/L (nominal concentrations) were obtained for the endpoint respiration inhibition after 30 minutes and 3 hours of exposure, respectively. The 3-hour EC50 value of 135 mg/L was used for the PNEC STP derivation of C12-14AS TEA (CAS 90583-18-9).

Moreover, a GLP test according to DIN 38412 part 8 with Pseudomonas putida using C12-14AS TEA (CAS 90583-18-9) is available (Guhl 1992). In this study, the growth inhibition of Pseudomonas putida was recorded after approximately 16 hours of exposure. The 16-h EC10 value was determined to be 1084 mg a.i./L (nominal concentration). Since performed with single species, this test was chosen to be a supporting study. The second supporting study with C12 -14AS TEA (CAS 90583-18-9) was preformed accordin to DIN 38412, part 27. The 30 min EC0 value of > 4090 mg a.i./L was reported based on the respiration rate of Pseudomonas putida.