Fatty acids, C16-18, reaction products with triethanolamine, di-Me sulfate-quaternized

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Remarks:

no analytical monitoring

Qualifier:

according to guideline

Guideline:

DIN 38412-27 (Pseudomonas putida Zellvermehrungshemmtest)

Version / remarks:

Nov. 1992

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Preparation of a slight turbid stock suspension with nominal concentration of 1000 mg product/l dilution water. The corresponding volumes were taken under permanent stirring and pipetting into the test vessels.

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

30 min

Hardness:

-

Test temperature:

20.8 - 21.0 °C

pH:

-

Dissolved oxygen:

-

Salinity:

-

Nominal and measured concentrations:

Nominal concentrations: control, 10, 30, 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: 100 ml Erlenmeyer flask
- Fill volume: 100 ml
- Aeration: directly
- No. of vessels per concentration (replicates): 2
- No. of blank values (replicates): 4
- Date of test: 1998-04-08
-Age of bacteria suspension: 2 days

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water according to DIN 38412, part 27, 8.1, deviation: stock solution contained 33.3 mg Na2HPO4 x H2O /l and 0.5 g NH4Cl/l. To dilution water aliquots of test substance solution and 2 ml glucose solution and 1-3 ml bacterial suspenson were added.

Reference substance (positive control):

no

Key result

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

60 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

EC0

Effect conc.:

24 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

29.6 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

Results on nominal effect concentrations (80 % a.i.)
30 min EC50: 75 mg/L, test mat.
30 min EC0: 30 mg/L, test mat.
30 min EC10: 37 mg/L, test mat.

Results on nominal effect concentrations (acitve ingredient)
30 min EC50: 60 mg/L, nominal
30 min EC0: 24 mg/L, nominal
30 min EC10: 29.6 mg/L, nominal

Table 1: O2 decrease after 30 minutes

blank value	Conc. mg prod./l	Replicate	mg O2/(l x min)
1			1.73
2			1.83
	10	Replicate 1	1.83
		Replicate 2	1.81
	30	Replicate 1	1.71
		Replicate 2	1.72
	100	Replicate 1	0.52
		Replicate 2	0.54
3			1.79
4			1.84

 

Table 2: Inhibition of O2 decrease (mean values related of blank values)

Conc. mg prod./l	mean value inhibition (+), increase (-) form 2 replicates
10	- 1.1
30	4.6
100	71

Validity criteria fulfilled:

yes

Conclusions:

In this study with test substance fully saturated TEA-Esterquat the acute bacterial toxcitiy was determined according to DIN 38412, part 27 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest). The calculated effect concentrations after 30 minutes were as follows: EC0: 24.0 mg a.i./l, EC10: 29.6 mg a.i./l, EC50: 60 mg a.i./l.
Sample preparation and testing process following close after the other. We assume that hydrolysis can be neglected due to the short duration of test (30 min). Therefore the nominal concentration can be accepted as effect concentration, and reliability can be assigned as 2.

Executive summary:

In a study with test substance fully saturated TEA-Esterquat the acute bacterial toxicity towards Pseudomonas putida was determined under static conditions according to DIN 38412, part 27 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest) at test material concentrations of 10, 30, 100 mg test mat./L. The calculated effect concentrations after 30 minutes were as follows: EC0: 24.0 mg a.i./l, EC10: 29.6 mg a.i./l, EC50: 60 mg a.i./l.

This toxicity study is classified as reliable with restrictions and satisfies the guideline requirements for a respiration inhibition test according to DIN 38412, part 27.

 

Results Synopsis

 

Test Organism:Pseudomonas putida

Test Type (Static): DIN 38412, part 27.

 

30 min EC0: 24.0mg a.i./L

30 min EC10: 29.6 mg a.i./L

30 min EC50: 60 mg a.i./L

 

 

Endpoint(s) Effected:  Respiration rate

Description of key information

30 min EC10 = 29.6 mg a.i./L; 30 min EC50 = 60 mg a.i./L (DIN 38412, part 27); GLP, RL1

Key value for chemical safety assessment

EC50 for microorganisms:

60 mg/L

EC10 or NOEC for microorganisms:

29.6 mg/L

Additional information

A bacterial toxicity study is available for the target substance fully saturated TEA-Esterquat. In addition, supporting studies are available for the structurally related source substance partially unsaturated TEA-Esterquat.

In a study with fully saturated TEA-Esterquat the acute bacterial toxicity towards Pseudomonas putida was determined under static conditions according to DIN 38412, part 27 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest) at test material concentrations of 10, 30, 100 mg test mat./L. The calculated effect concentrations after 30 minutes were as follows: EC0: 24.0 mg a.i./L, EC10: 29.6 mg a.i./L, EC50: 60 mg a.i./L.

 

Supporting data are available for partially unsaturated TEA-Esterquat:

In a 3 hour toxicity study conducted according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test) and EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test), the cultures of activated sludge of a predominantly domestic sewage treatment plant were exposed to partially unsaturated TEA-Esterquat (CAS No. 157905-74-3) at nominal concentrations of 0, 1, 3, 9, 27, 81, 243 mg/L under static conditions. There was no inhibitory effect of the test item at any concentration; the deviations from the untreated controls were less than 15 %, both after 30 min and 3 hours. The 3 h EC50 value based on inhibition of respiration was > 243 mg/L.

 

The toxic effect of partially unsaturated TEA-Esterquat on Pseudomonas putida was assessed by measuring the respiration rate under defined conditions in the presence of different concentrations of partially unsaturated TEA-Esterquat (solvent free, 100 % a.i.) according to DIN 38412, part 27, 1992 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest)) at nominal concentrations of control, 10, 30 100, 300 1000, 3000, 10000 mg a.i./L. The calculated effect concentrations were as follows: 30 min EC0: 30 mg/L, 30 min EC10: 35 mg/L, and 30 min EC50 110 mg/L.

 

The toxic effect of partially unsaturated TEA-Esterquat on activated sludge from a biological treatment plant was assessed by measuring the respiration rate under defined conditions in the presence of different concentrations of the test article. According to OECD guideline 209 a concentration range between 16 and 125.0 mg/L of the test article was used in the test. Concentrations of 250 mg/L and 125 mg/L supported the respiration rates. Values of 2.6 % and 17.9 % compared to the untreated controls were obtained. Inhibition of the respiration rates were obtained with concentrations of 62, 32, and 16 mg/L of the test article and were determined to be 2.6 %, 7.7 %, and 7.7 %, respectively, compared to the untreated control. From the results, it was concluded to state that the test article did not severely inhibit the respiration rate of activated sludge obtained from a biological treatment plant. The stock solution of the test substance was prepared immediately before the test. The EC50 of the test substance is higher than 250 mg/L.