Fatty acids, C16-18, reaction products with triethanolamine, di-Me sulfate-quaternized

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

REPORTING FORMAT FOR THE ANALOGUE APPROACH

1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the hypothesis that source and target substances have similar (eco)toxicological properties because
• they share structural similarities with common functional groups: One quaternised ethanolamine moiety, one to three, mainly two ester groups with a typical UVCB distribution with long-chain fatty acids of natural origin. The molecular structure is almost identical.
• they are manufactured from similar resp. identical precursors (triethanolamine, long-chain fatty acids, dimethyl sulphate) under similar conditions. Therefore, common breakdown products via physical and biological processes, which result in structurally similar chemicals are evident
• A constant pattern in the changing of the potency of the properties across the TEA-Esterquats by chain-length and the grade of esterification is not observed, because the fatty acid chain-length distribution is too narrow and similar and the distribution of mono-, di-, and tri-esters is identical. Some variation caused by variation in C=C double bonds may occur and will be discussed at the relevant endpoint.

2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
See justification for read-across attached to chapter 13 of this IUCLID file.

3. ANALOGUE APPROACH JUSTIFICATION
See justification for read-across attached to chapter 13 of this IUCLID file.

4. DATA MATRIX
See justification for read-across attached to chapter 13 of this IUCLID file.

Reason / purpose for cross-reference:

read-across: supporting information

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Test organisms (species):

Daphnia magna

Total exposure duration:

4 h

Test temperature:

20.2 - 20.3 °

Salinity:

-

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.23 mg/L

Nominal / measured:

estimated

Conc. based on:

other: corrected for recovery of 93%

Basis for effect:

other: immobilisation

Remarks on result:

other: a.i. 100 %

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

ca. 0.93 mg/L

Nominal / measured:

estimated

Conc. based on:

other: corrected for rec overy of 93%

Basis for effect:

other: immobilisation

Remarks on result:

other: a.i. 100 %

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

ca. 4.46 mg/L

Nominal / measured:

estimated

Conc. based on:

other: corrected for recovery of 93%

Basis for effect:

other: immobilisation

Remarks on result:

other: a.i. 100 %

Details on results:

Results of estimated effect concentration (basis for effect: immobilisation):
48 h EC50: 2.23 mg/L, corrected for recovery of 93%
48 h EC0: ca. 0.93 mg/L, corrected for rec overy of 93%
48 h EC100: ca. 4.46 mg/L, corrected for recovery of 93%

Results of nominal effect concentration (basis for effect: immobilisation):
48 h EC50: 2.4 mg/L, test mat., recovery in average 93 %
48 h EC0: 1 mg/L, test mat., recovery in average 93 %
48 h EC100: 4.8 mg/L, test mat.,recovery in average 93 %

- The average recovery rate was 93% of nominal concentration.
- No abnormal behaviour during test.
- Slight turbidity was observed at the two hightest test concentration (51.5, 113 mg/l)

Validity criteria fulfilled:

yes

Conclusions:

In this study the 48 h EC 50 of the analogue source test substance "partially unsaturated TEA-Esterquat" (solvent free) for Daphnia magna was 2.23 mg/l based on estimated concentration, corrected for recovery of 93% . The recovery rate was > 80 % of nominal concentration throughout the whole test.

Description of key information

48 h EC50 = 2.23 mg a.i./L (EU method C.2, Daphnia magna); GLP; RL1; read-across partially unsaturated TEA-Esterquat

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

2.23 mg/L

Additional information

One short-term toxicity study is available for the target substance fully saturated TEA-Esterquat. In addition, short-term toxicity studies with Daphnia are available for the source substance partially unsaturated TEA-Esterquat. A justification for read-across is attached to Iuclid section 13.

 

In an acute toxicity study according to EU method C.2 the estimated 48 h EC 50 of the test substance fully saturated TEA-Esterquat was 0.53 mg/l for Daphnia magna. The effect concentrations were corrected because the recovery rate was < 80 % of nominal concentration at 0 and 24 hours. The reliability 3 was assigned to this study because the results of the analytical control were not reliable. The substance recovery was low and not reproducible.

 

The 48 hr acute toxicity of partially unsaturated TEA-Esterquat (solvent free) to water flea (Daphnia magna) according to EU Method C.2 (Acute Toxicity for Daphnia) was studied under static conditions.  Daphnids were exposed to control, and partially unsaturated TEA-Esterquat (solvent free) at nominal concentration of 1, 2.2, 4.84, 10.6, 23.4, 51.5, 113 mg test mat./L for 48 hr.  Mortality/immobilization was observed daily. The 48 hour EC50 was 2.23 mg a.i./L. The effect concentration was corrected for the analytical recovery of 93%.

 

In a supporting study, the 48 hr acute toxicity of partially unsaturated TEA-Esterquat (CAS No. 157905-74-3) was investigated in a study conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Under static conditions Daphnia magna were exposed to control and test chemical at nominal concentration of 0.2, 0.44, 0.97, 2.13, 4.69, 10.3, 22.7 mg/L (mean measured concentration: 0.09, 0.2, 0.43, 0.95, 2.1, 4.6, 10.1 mg/L). The mean concentrations during the test (mean of samples taken at 0 h and 48 h) were between 34.7 % and 51.7 % of nominal (average 44.5 %). Immobilization and sublethal effects were observed daily. The 48 hour EC50 based on mean measured concentrations was 6.05 mg/L.

 

The lowest 48 h EC50 in Daphnia was 2.23 mg a.i./L.