Registration Dossier
Registration Dossier
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EC number: 283-406-2 | CAS number: 84625-32-1 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Eucalyptus globulus, Myrtaceae.
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Constituent approach, additivity formula:
48h-EC50 for aquatic invertebrates = 1.02 mg/L for Eucalyptus oil.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1.02 mg/L
Additional information
Based on a weight of evidence approach, the acute toxicity of Eucalyptus oil to aquatic invertebrates can be estimated using adequate toxicity data of major constituents, greater than 10% in the mixture. Three constituents were considered, representing 90% of the mixture. To obtain a worst case estimation, the worst case percentages of each constituent were taken into account based on the individual toxicities of these compounds. These data are summarized in the table below:
Constituents |
Worst-case composition |
Aquatic invertebrates 48h-EC50, mg/L |
Reference in IUCLID 6 |
Cineol 1,8 |
55% |
> 100 |
1,8-Cineol, 470-82-6, Short-term toxicity to aquatic invertebrates, Vryenhoef, 2012, RS, W |
Limonene |
12% |
0.307 |
(d)-limonene, 5989-27-5, Short-term toxicity to aquatic invertebrates, Betat, 2013, RS, W |
Pinene α |
23% |
0.475 |
alpha-pinene, 7785-26-4, Short-term toxicity to aquatic invertebrates, Betat, 2013, RS, W |
The acute toxicity of Eucalyptus oil to aquatic invertebrates was estimated using the following additivity formula, as recommended in the Regulation (EC) No 1272/2008 (CLP):
∑ Ci / L(E)C50m = ∑ (Ci / L(E)C50i)
Where,
Ci = concentration of component i (weight percentage);
L(E)C50i = LC50 or EC50 for component i, in mg/L;
L(E)C50m = L(E)C50 of the part of the mixture with test data;
Based on data presented above, the 48h-EC50m value for Eucalyptus oil was estimated to be 1.02 mg/L for aquatic invertebrates for a worst case typical mixture. The worst case assumption is validated with the comparable calculations performed on fishes and validated with 2 studies.
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