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Short-term toxicity to aquatic invertebrates

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Description of key information

Constituent approach, additivity formula:
48h-EC50 for aquatic invertebrates = 1.02 mg/L for Eucalyptus oil.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
1.02 mg/L

Additional information

Based on a weight of evidence approach, the acute toxicity of Eucalyptus oil to aquatic invertebrates can be estimated using adequate toxicity data of major constituents, greater than 10% in the mixture. Three constituents were considered, representing 90% of the mixture. To obtain a worst case estimation, the worst case percentages of each constituent were taken into account based on the individual toxicities of these compounds. These data are summarized in the table below:

Constituents

Worst-case composition

Aquatic invertebrates

48h-EC50, mg/L

Reference in IUCLID 6

Cineol 1,8

55%

> 100

1,8-Cineol, 470-82-6, Short-term toxicity to aquatic invertebrates, Vryenhoef, 2012, RS, W

Limonene

12%

0.307

(d)-limonene, 5989-27-5, Short-term toxicity to aquatic invertebrates, Betat, 2013, RS, W

Pinene α

23%

0.475

alpha-pinene, 7785-26-4, Short-term toxicity to aquatic invertebrates, Betat, 2013, RS, W

The acute toxicity of Eucalyptus oil to aquatic invertebrates was estimated using the following additivity formula, as recommended in the Regulation (EC) No 1272/2008 (CLP):

∑ Ci / L(E)C50m = ∑ (Ci / L(E)C50i)

Where,

Ci = concentration of component i (weight percentage);

L(E)C50i = LC50 or EC50 for component i, in mg/L;

L(E)C50m = L(E)C50 of the part of the mixture with test data;

 

Based on data presented above, the 48h-EC50m value for Eucalyptus oil was estimated to be 1.02 mg/L for aquatic invertebrates for a worst case typical mixture. The worst case assumption is validated with the comparable calculations performed on fishes and validated with 2 studies.