Eucalyptus globulus, ext.

Short-term toxicity to aquatic invertebrates

Description of key information

Constituent approach, additivity formula:
48h-EC50 for aquatic invertebrates = 1.02 mg/L for Eucalyptus oil.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1.02 mg/L

Additional information

Based on a weight of evidence approach, the acute toxicity of Eucalyptus oil to aquatic invertebrates can be estimated using adequate toxicity data of major constituents, greater than 10% in the mixture. Three constituents were considered, representing 90% of the mixture. To obtain a worst case estimation, the worst case percentages of each constituent were taken into account based on the individual toxicities of these compounds. These data are summarized in the table below:

Constituents	Worst-case composition	Aquatic invertebrates 48h-EC50, mg/L	Reference in IUCLID 6
Cineol 1,8	55%	> 100	1,8-Cineol, 470-82-6, Short-term toxicity to aquatic invertebrates, Vryenhoef, 2012, RS, W
Limonene	12%	0.307	(d)-limonene, 5989-27-5, Short-term toxicity to aquatic invertebrates, Betat, 2013, RS, W
Pinene α	23%	0.475	alpha-pinene, 7785-26-4, Short-term toxicity to aquatic invertebrates, Betat, 2013, RS, W

The acute toxicity of Eucalyptus oil to aquatic invertebrates was estimated using the following additivity formula, as recommended in the Regulation (EC) No 1272/2008 (CLP):

∑ Ci / L(E)C50m = ∑ (Ci / L(E)C50i)

Where,

Ci = concentration of component i (weight percentage);

L(E)C50i = LC50 or EC50 for component i, in mg/L;

L(E)C50m = L(E)C50 of the part of the mixture with test data;

 

Based on data presented above, the 48h-EC50m value for Eucalyptus oil was estimated to be 1.02 mg/L for aquatic invertebrates for a worst case typical mixture. The worst case assumption is validated with the comparable calculations performed on fishes and validated with 2 studies.